Nickavar, B., Rezaee, J., Nickavar, A. (2016). Effect-Directed Analysis for the Antioxidant Compound in Salvia verticillata. Iranian Journal of Pharmaceutical Research, 15(1), 241-246. doi: 10.22037/ijpr.2016.1793
Bahman Nickavar; Javad Rezaee; Azar Nickavar. "Effect-Directed Analysis for the Antioxidant Compound in Salvia verticillata". Iranian Journal of Pharmaceutical Research, 15, 1, 2016, 241-246. doi: 10.22037/ijpr.2016.1793
Nickavar, B., Rezaee, J., Nickavar, A. (2016). 'Effect-Directed Analysis for the Antioxidant Compound in Salvia verticillata', Iranian Journal of Pharmaceutical Research, 15(1), pp. 241-246. doi: 10.22037/ijpr.2016.1793
Nickavar, B., Rezaee, J., Nickavar, A. Effect-Directed Analysis for the Antioxidant Compound in Salvia verticillata. Iranian Journal of Pharmaceutical Research, 2016; 15(1): 241-246. doi: 10.22037/ijpr.2016.1793
Effect-Directed Analysis for the Antioxidant Compound in Salvia verticillata
1Department of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences
2Department of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3Aliasghar Children’s Hospital, Iran University of Medical sciences, Tehran, Iran
Abstract
Salvia genus is one of the largest genera of the Lamiaceae family. Its species have been used for a wide variety of disorders in the local traditional medicine systems. Therefore, the genus has been the subject of several phytochemical and biological studies. The aim of the study was to identify the major antioxidant compound(s) from the methanol extract of Salvia verticillata using activity-guided fractionation. The crude extract showed strong antioxidant activities in DPPH and β-carotene/linoleic acid tests. The ethyl acetate fraction also exhibited a potent free radical scavenging activity compared to the other fractions. Further fractionation and purification of the ethyl acetate fraction using chromatography methods yielded a compound with high antioxidant capacity. The isolated active compound was determined as chrysoeriol. It showed a dose-dependent free radical scavenging activity with an IC50 (DPPH scavenging) value of 93.32 (80.23 – 108.57) mM.
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