Characterization of Encapsulated Berberine in Yeast Cells of Saccharomyces cerevisiae

Document Type: Research article

Authors

1 Department of Drug and Food Control, School of Pharmacy, Mashhad University of Medical Sciences.

2 Department of Drug and Food Control, School of Pharmacy, Mashhad University of Medical Sciences.Targetted Drug Delivery Research Centre, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

3 Department of Drug and Food Control, School of Pharmacy, Mashhad University of Medical Sciences

4 Department of Veterinary Research and Biotechnology, Razi Vaccine and Serum Research Institute, Mashhad, Iran

5 Department of Drug and Food Control, School of Pharmacy, Mashhad University of Medical Sciences.Biotechnology Research Centre, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

Abstract

Berberine was loaded in yeast cells of Saccharomyces cerevisiaeas a novel pharmaceutical carrier to improve the treatment ofmany diseases. The yeast-encapsulated active materialsshowedhigh stability and bioavailability due to the enhanced solubility and sustained releasing. In this study, different characteristics of prepared berberine loaded yeast cells (loading capacity, release kinetic order, MIC and stability) were evaluatedby different analytical methods (fluorescence spectroscopy, HPLC and SEM).The loading capacity was about 78% ± 0.6%.Berberine release patterns of microcapsules happened in two different stages and followed by zero and first-order kinetic,respectively. About 99% of all active material released during 34 h. MIC was improved by berberine loaded microcapsules in comparison withberberine powder. The microcapsules were completely stable. Berberine loaded Sac. Cerevisiae could be considered as a favorite sustained release drug delivery system. The yeast would be applied as an efficient carrier to improve various properties of different active materials.

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