Synthesis, Antiplatelet Activity and Cytotoxicity Assessment of Indole-Based Hydrazone Derivatives

Haj Mohammad Ebrahim Tehrani, Kamaleddin; Esfahani Zadeh, Marjan; Mashayekhi, Vida; Hashemi, Maryam; Kobarfard, Farzad; Gharebaghi, Farhad; Mohebbi, Shohreh

doi:10.22037/ijpr.2015.1739

Synthesis, Antiplatelet Activity and Cytotoxicity Assessment of Indole-Based Hydrazone Derivatives

Document Type : Research article

Authors

Kamaleddin Haj Mohammad Ebrahim Tehrani ¹
Marjan Esfahani Zadeh ²
Vida Mashayekhi ³
Maryam Hashemi ⁴
Farzad Kobarfard ²
Farhad Gharebaghi ⁴
Shohreh Mohebbi ⁵

¹ Zanjan Pharmaceutical Biotechnology Research Center

² Department of Medicinal Chemistry, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

³ Department of Pharmaceutical Biotechnology, School of Pharmacy, Zanjan University of Medical Sciences. Zanjan, Iran

⁴ Department of Medicinal Chemistry, School of Pharmacy, Zanjan University of Medical Sciences. Zanjan, Iran

⁵ Department of medicinal chemistry, school of pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.

10.22037/ijpr.2015.1739

Abstract

A series of indole-based aryl(aroyl)hydrazone analogs of antiplatelet indole-3-carboxaldehyde phenylhydrazone were synthesized by the Schiff base formation reaction and their antiplatelet activity was assessed using human platelet rich plasma. The platelet concentrate was obtained using a two-step centrifugation protocol and ADP, arachidonic acid and collagen were used as inducers of platelet aggregation. Based on the results, substituted phenylhydrazones showed promising activity. Among them, compound 1i was the most potent derivative with an IC₅₀ comparable to that of indomethacin as a standard drug. The hydrazone derivatives were also tested for their cytotoxicity using on platelet concentrates and fibroblast L929 cells. The majority of the derivatives showed an acceptable selectivity towards antiplatelet aggregation activity.
Based on the activity data, phenylhydrazone derivatives (1a-i) exhibited considerable antiplatelet activity and minimal toxic effect on platelet cells. The results of the present study could provide a better understanding of the structure activity relationship of antiplatelet indolehydrazones.

Keywords