Biotransformation of Progesterone by Whole Cells of Filamentous Fungi Aspergillus brasiliensis

Document Type: Research article

Authors

1 aDepartment of pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

2 Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3 Department of pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

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Abstract

Microbial steroid biotransformation have found wide-reaching application for the production of more precious and functionalized compounds due to their high regio-and stereoselectivity.
In this study, the possibility of using filamentous fungi Aspergillus brasiliensis cells in the biotransformation of progesterone (I), a C-21 steroid hormone was studied for the first time.
The fungal strain was inoculated into the transformation medium which supplemented with progesterone as a substrate. Biotransformation of this steroid for 7 days afforded 3 different hydroxylated metabolites: 11α-hydroxyprogesterone (II); 14α-hydroxyprogesterone (III) and 21-hydroxyprogesterone (IV).
The metabolites were separated by thin layer chromatography. Structure determinations of the metabolites were performed by comparing NMR, MS and IR spectra of starting compound with those of metabolites.
These results may be of industrial importance because the metabolites can be used as precursor of some steroid drugs.

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