• Home
  • Browse
    • Current Issue
    • By Issue
    • By Author
    • By Subject
    • Author Index
    • Keyword Index
  • Journal Info
    • About Journal
    • Aims and Scope
    • Editorial Board
    • Publication Ethics
    • Indexing and Abstracting
    • Related Links
    • FAQ
    • Peer Review Process
    • News
  • Guide for Authors
  • Submit Manuscript
  • Reviewers
  • Contact Us
 
  • Login
  • Register
Home Articles List Article Information
  • Save Records
  • |
  • Printable Version
  • |
  • Recommend
  • |
  • How to cite Export to
    RIS EndNote BibTeX APA MLA Harvard Vancouver
  • |
  • Share Share
    CiteULike Mendeley Facebook Google LinkedIn Twitter
Iranian Journal of Pharmaceutical Research
arrow Articles in Press
arrow Current Issue
Journal Archive
Volume Volume 18 (2019)
Volume Volume 17 (2018)
Volume Volume 16 (2017)
Volume Volume 15 (2016)
Volume Volume 14 (2015)
Issue Issue 4
Issue Issue 3
Issue Issue 2
Issue supplement
Issue Issue 1
Volume Volume 13 (2014)
Volume Volume 12 (2013)
Volume Volume 11 (2012)
Volume Volume 10 (2011)
Volume Volume 9 (2010)
Volume Volume 8 (2009)
Volume Volume 7 (2008)
Volume Volume 6 (2007)
Volume Volume 5 (2006)
Volume Volume 4 (2005)
Volume Volume 3 (2004)
Volume Volume 2 (2003)
Volume Volume 1 (2002)
Daeihamed, M., Haeri, A., Dadashzadeh, S. (2015). A Simple and Sensitive HPLC Method for Fluorescence Quantitation of Doxorubicin in Micro-volume Plasma: Applications to Pharmacokinetic Studies in Rats. Iranian Journal of Pharmaceutical Research, 14(supplement), 33-42. doi: 10.22037/ijpr.2015.1710
Marjan Daeihamed; Azadeh Haeri; Simin Dadashzadeh. "A Simple and Sensitive HPLC Method for Fluorescence Quantitation of Doxorubicin in Micro-volume Plasma: Applications to Pharmacokinetic Studies in Rats". Iranian Journal of Pharmaceutical Research, 14, supplement, 2015, 33-42. doi: 10.22037/ijpr.2015.1710
Daeihamed, M., Haeri, A., Dadashzadeh, S. (2015). 'A Simple and Sensitive HPLC Method for Fluorescence Quantitation of Doxorubicin in Micro-volume Plasma: Applications to Pharmacokinetic Studies in Rats', Iranian Journal of Pharmaceutical Research, 14(supplement), pp. 33-42. doi: 10.22037/ijpr.2015.1710
Daeihamed, M., Haeri, A., Dadashzadeh, S. A Simple and Sensitive HPLC Method for Fluorescence Quantitation of Doxorubicin in Micro-volume Plasma: Applications to Pharmacokinetic Studies in Rats. Iranian Journal of Pharmaceutical Research, 2015; 14(supplement): 33-42. doi: 10.22037/ijpr.2015.1710

A Simple and Sensitive HPLC Method for Fluorescence Quantitation of Doxorubicin in Micro-volume Plasma: Applications to Pharmacokinetic Studies in Rats

Article 4, Volume 14, supplement, Winter 2015, Page 33-42  XML PDF (593.03 K)
Document Type: Research article
DOI: 10.22037/ijpr.2015.1710
Authors
Marjan Daeihamed1; Azadeh Haeri1; Simin Dadashzadeh email 2
1School of Pharmacy, Shahid Beheshti University of Medical Sciences
2Department of Pharmaceutics, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract
A validated HPLC method was developed to determine doxorubicin concentration in a small volume of rat plasma (60 µl) with convenient fluorescence detection. Sample preparation includes a simple one-step liquid-liquid extraction using minimum amount of organic solvent, with extraction recoveries more than 95%. The analysis was accomplished using PerfectSil C18 column maintained at 35 °C and a mobile phase consisted of acetonitrile and water (32:68, v/v; pH=2.6). The flow-rate was kept at 1 mL/min and column effluent was monitored with a fluorescence detector at an excitation and emission wavelength of 470 and 555 nm, respectively. The detection limit was 5 ng/mL. No analytical interference was observed from endogenous components in rat plasma. This method was feasibly applied to the pharmacokinetic study of 5 mg/kg of doxorubicin after intravenous administration to rats.
Keywords
doxorubicin; HPLC; Fluorescence; Pharmacokinetics; Rat plasma
Main Subjects
Drug quality control; Medicinal chemistry; Pharmacutics
Statistics
Article View: 4,965
PDF Download: 18,459
Home | Glossary | News | Aims and Scope | Sitemap
Top Top

Statistics

Journal Management System. Designed by sinaweb.