Naghibione; A Novel Sesquiterpenoid with Antiplasmodial Effect from Dorema hyrcanum Koso-Pol. Root, a Plant Used in Traditional Medicine

Document Type: Research article

Authors

1 1. Traditional Medicine and Materia Medica Research Center (TMRC), Shahid Beheshti University of Medical Sciences; 2. School of Pharmacy Shahid Beheshti University.

2 1. Traditional Medicine and Materia Medica Research Center (TMRC), Shahid Beheshti University of Medical Sciences; 2. Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences.

3 1. School of traditional Medicine 2. Traditional Medicine & Materia Medica Research Center Shahid Beheshti University of Medical Sciences

4 Institut fuer Pharmazie (Pharmazeutische Biologie), Freie Universitaet Berlin

Abstract

Some Dorema species are used in Persian traditional medicine. In the present study the total extract from the roots of Dorema hyrcanum Koso-Pol. was investigated for its in vitro (pLDH assay) and in vivo (Peters’ 4-days suppressive test) antiplasmodial effects and assessed for cytotoxicity against the normal cell line MDBK (MTT test). The IC50 values for a chloroquine- sensitive (3D7) and a chloroquine- resistance (K1) strain of Plasmodium falciparum were 28.64 and 9.79 µg/ml, respectively. The inhibition percentage of the rodent parasite, Plasmodium berghei, on day 4 in mice was 77.9% and IC50 value on Madin–Darby bovine kidney cells (MDBK cells) was 59.84µg/ml.
The total extract was subjected to a bioassay-guided fractionation protocol based on the in vivo model which resulted in the isolation of an acetophenon (compound 1), one new sesquiterpenoid, 1(2-hydroxy-4-methoxy)- 3,7,11- trimethyl-3-vinyl-6(E), 10 dodecadiene- 1- dione (compound 2) and two known sesquiterpenoid derivatives (compounds 3, 4). Their structures were elucidated by spectroscopic analysis, including 1D and 2D NMR experiments and ESI-MS. All compounds were evaluated for in vivo antiplasmodial effect and the results revealed that compound 2 showed good suppression activity, inhibiting 68.1 % of the parasite growth.

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