Molecular Mechanisms of Luteolin Induced Growth Inhibition and Apoptosis of Human Osteosarcoma Cells

Document Type: Research article

Authors

1 Department of Orthopedics, 86 Hospital of PLA, Dangtu, Anhui 243100, China

2 Department of Orthopedics, Japan Union Hospital of Jilin University, Changchun130033,China

3 Department of Orthopedics, 86 Hospital of PLA, Dangtu, Anhui 243100, China.

4 Department of Pharmacy, Jinling Hospital, Nanjing, Jiangsu 210002, China.

5 1Department of Orthopedics, Jingdu Hospital, Nanjing 210002, China 2Department of Orthopedics, 86 Hospital of PLA, Dangtu, Anhui 243100, China

Abstract

Luteolin is a flavone in medicinal plants as well as some vegetables and spices. It is a natural anti-oxidant with less pro-oxidant potential but apparently with a better safety profile. The purpose of this study was to investigate the molecular mechanism of luteolin-mediated apoptosis of MG-63 human osteosarcoma cells. MTT assay kit was employed to evaluate the effects of luteolin on MG-63 cells proliferation. Then, we performed Annexin V-FITC/PI to analyze the apoptotic rate of the cells. Furthermore, the inhibitory effects of luteolin on the expressions of BCL-2, BAX, Caspase-3 and Survivin were detected by Western blotting. As expected, luteolin (0.5, 2.5, 12.5 µg/ml) inhibited the growth of MG-63 cells by inhibiting cell proliferation and inducing cell apoptosis. Western blotting demonstrated that luteolin (0.5, 2.5, 12.5 µg/ml) inhibited the expressions of BCL-2, Caspase-3 and Survivin, and promoted the expression of BAX in MG-63 cells with a concentration dependent way. Luteolin can inhibit osteosarcoma cell proliferation and induce apoptosis effectively in a dose dependent manner through down-regulating the expression of BCL-2, Caspase-3 and Survivin proteins levels and up-regulating the expression of BAX protein level. These findings indicated that luteolin may be used as a novel herbal medicine for the treatment of osteosarcoma.

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