Document Type: Research article
Department of Orthopedics, 86 Hospital of PLA, Dangtu, Anhui 243100, China
of Orthopedics, Japan Union Hospital of Jilin University, Changchun130033,China
Department of Orthopedics, 86 Hospital of PLA, Dangtu, Anhui 243100, China.
Department of Pharmacy, Jinling Hospital, Nanjing, Jiangsu 210002, China.
1Department of Orthopedics, Jingdu Hospital, Nanjing 210002, China 2Department of Orthopedics, 86 Hospital of PLA, Dangtu, Anhui 243100, China
Luteolin is a flavone in medicinal plants as well as some vegetables and spices. It is a natural anti-oxidant with less pro-oxidant potential but apparently with a better safety profile. The purpose of this study was to investigate the molecular mechanism of luteolin-mediated apoptosis of MG-63 human osteosarcoma cells. MTT assay kit was employed to evaluate the effects of luteolin on MG-63 cells proliferation. Then, we performed Annexin V-FITC/PI to analyze the apoptotic rate of the cells. Furthermore, the inhibitory effects of luteolin on the expressions of BCL-2, BAX, Caspase-3 and Survivin were detected by Western blotting. As expected, luteolin (0.5, 2.5, 12.5 µg/ml) inhibited the growth of MG-63 cells by inhibiting cell proliferation and inducing cell apoptosis. Western blotting demonstrated that luteolin (0.5, 2.5, 12.5 µg/ml) inhibited the expressions of BCL-2, Caspase-3 and Survivin, and promoted the expression of BAX in MG-63 cells with a concentration dependent way. Luteolin can inhibit osteosarcoma cell proliferation and induce apoptosis effectively in a dose dependent manner through down-regulating the expression of BCL-2, Caspase-3 and Survivin proteins levels and up-regulating the expression of BAX protein level. These findings indicated that luteolin may be used as a novel herbal medicine for the treatment of osteosarcoma.