Development of a Rapid Derivative Spectrophotometric Method for Simultaneous Determination of Acetaminophen, Diphenhydramine and Pseudoephedrine in Tablets

Document Type: Research article

Authors

1 -

2 Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Research Center, Tehran University of Medical Sciences, Tehran (14155-6451), Iran

Abstract

A mixture of acetaminophen, diphenhydramine hydrochloride and pseudoephedrine
hydrochloride is used for the symptomatic treatment of common cold. In this study, a
derivative spectrophotometric method based on zero-crossing technique was proposed
for simultaneous determination of acetaminophen, diphenhydramine hydrochloride and
pseudoephedrine hydrochloride. Determination of these drugs was performed using the 1D
value of acetaminophen at 281.5 nm, 2D value of diphenhydramine hydrochloride at 226.0 nm
and 4D value of pseudoephedrine hydrochloride at 218.0 nm.
The analysis method was linear over the range of 5-50, 0.25-4, and 0.5-5 μg/mL for
acetaminophen, diphenhydramine hydrochloride and pseudoephedrine hydrochloride,
respectively. The within-day and between-day CV and error values for all three compounds
were within an acceptable range (CV

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