Fatty Acid and Essential Oil Compositions of Trifolium angustifolium var. angustifolium with Antioxidant, Anticholinesterase and Antimicrobial Activities
1Dept of Pharmacognosy, Faculty of Pharmacy, Dicle University, 21280 Diyarbakir, Turkey
2Dept of General and Analytical Chemistry, Faculty of Pharmacy, Istanbul University, 34116 Beyazıt, Istanbul, Turkey
3Dept of Nutrition and Dietetics, Sch of Health, Batman University, 72060, Batman, Turkey
4Research and Application of Science and Technology Center (DÜBTAM), Dicle University, 21280 Diyarbakir, Turkey
Abstract
This study represents the first report on the chemical composition and biological activity of Trifolium angustifolium var. angustifolium. The major components of the essential oil were identified as hexatriacontene (23.0%), arachidic acid (15.5%) and α-selinene (10.0%). The main constituents of the fatty acid obtained from the petroleum ether extract were identified as palmitic acid (29.8%), linoleic acid (18.6%) and oleic acid (10.5%). In particular, the water extract exhibited higher activity than α-tocopherol and BHT, which were used as standards in the ABTS cation radical scavenging assay and indicated higher inhibitory effect against acetylcholinesterase enzyme than the reference compound, galanthamine but exhibited weak activity in β-carotene bleaching, DPPH-free radical scavenging, and cupric-reducing antioxidant capacity assays. The petroleum ether extract exhibited higher activity than α-tocopherol which was used as standard in the β-carotene bleaching method at concentration 100 μg/mL. The acetone extract exhibited higher activity than α-tocopherol which was used as standard cupric reducing antioxidant capacity (CUPRAC) method at concentration 100 μg/mL. The acetone and methanol extracts were active on all microorganisms tested with a small zone diameter indicating weak activity.
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