Preparation and Characterization of Rifampin Loaded Mesoporous Silica Nanoparticles as a Potential System for Pulmonary Drug Delivery

Document Type: Research article

Authors

1 Department of Pharmaceutics, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

2 Department of Pharmaceutics, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Abstract

The goal of this research is to determine the feasibility of loading rifampin into mesoporous silica nanoparticles. Rifampin was selected as a model lipophilic molecule since it is a well-documented and much used anti tuberculosis drug. The mesoporous silica nanoparticles were prepared by using Tetraethyl ortho silicate and cetyltrimethyl ammonium bromide (as surfactant); The prepared nanoparticles were characterized in terms of their particle size measurement and porosimetry. The results showed that the particle size is 218±46 nm (mean± SD) and surface area is 816 m2g-1 . In order to load rifampin within the mesopores, adsorption experiments using three different solvents (methanol, water and dimethyl sulfoxide) were carried out. The loading procedure results in a significant improvement of the amount of rifampin loaded into mesoporous silica nanoparticles and methanol was found to be a suitable solvent providing a drug entrapment efficiency of 52 %. Rifampin loaded nanoparticles underwent different in-vitro tests including: SEM and Drug release. The in vitro drug release was investigated with buffer phosphate (pH=7.4). Regarding the drug release study a biphasic pattern of release was observed. The drug-loaded mesoporous silica nanoparticles was capable of releasing 100% drug content after 24h following a burst effect in the first four hours. The prepared rifampin loaded nanoparticles seem to have potential for use in a pulmonary drug delivery.

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