Size Reproducibility of Gadolinium Oxide Based Nanomagnetic Particles for Cellular Magnetic Resonance Imaging: Effects of Functionalization, Chemisorption and Reaction Conditions

Document Type: Research article

Authors

1 School of Medicine, Tehran University of Medical Sciences (TUMS

2 2Pharmaceutical Department, Food & Drug Laboratory Research Center, Food & Drug Organization (FDO), Ministry of Health

3 3Medical Physics & Biomedical Engineering Department, School of Medicine, Tehran University of Medical Sciences (TUMS)

Abstract

We developed biofunctionalized nanoparticles with magnetic properties by immobalizing diethyle-neglycol (DEG) on Gd2O3, and PEGilation of small particulate gadolinium oxide (SPGO) with two me-thoxy-polyethyleneglycol-silane (mPEG-Silane 550 and 2000 Da) using a new supervised polyol route, described recently. In conjunction to the previous study to achieve a high quality synthesis and increase in the product yield of nanoparticles; assessment of the effects of functionalization, chemisorption and altered reaction conditions, such as NaOH concentration, temperature, reaction time and their solubility, on size reproducibility were determined as the goals of this study. Moreover, the effects of centrifuga-tion, filtration and dialysis of the solution on the nonmagnetic particle size values and their stability against aggregation have been evaluated.
Optimization of reaction parameters led to strong coating of magnetic nanoparticles with the ligands which increases the reproducibility of particle size measurements. Furthermore, the ligand-coated nanoparticles showed enhanced coll