• Home
  • Browse
    • Current Issue
    • By Issue
    • By Author
    • By Subject
    • Author Index
    • Keyword Index
  • Journal Info
    • About Journal
    • Aims and Scope
    • Editorial Board
    • Editorial Staff
    • Publication Ethics
    • Indexing and Abstracting
    • Related Links
    • FAQ
    • Peer Review Process
    • News
  • Guide for Authors
  • Submit Manuscript
  • Reviewers
  • Contact Us
 
  • Login
  • Register
Home Articles List Article Information
  • Save Records
  • |
  • Printable Version
  • |
  • Recommend
  • |
  • How to cite Export to
    RIS EndNote BibTeX APA MLA Harvard Vancouver
  • |
  • Share Share
    CiteULike Mendeley Facebook Google LinkedIn Twitter Telegram
Iranian Journal of Pharmaceutical Research
Articles in Press
Current Issue
Journal Archive
Volume Volume 17 (2018)
Volume Volume 16 (2017)
Volume Volume 15 (2016)
Volume Volume 14 (2015)
Volume Volume 13 (2014)
Issue Issue 4
Issue Issue 3
Issue Issue 2
Issue Supplement
Issue Issue 1
Volume Volume 12 (2013)
Volume Volume 11 (2012)
Volume Volume 10 (2011)
Volume Volume 9 (2010)
Volume Volume 8 (2009)
Volume Volume 7 (2008)
Volume Volume 6 (2007)
Volume Volume 5 (2006)
Volume Volume 4 (2005)
Volume Volume 3 (2004)
Volume Volume 2 (2003)
Volume Volume 1 (2002)
Sahraroo, A., Babalar, M., Mirjalili, M., Fattahi Moghadam, M., Nejad Ebrahimi, S. (2014). In vitro Callus Induction and Rosmarinic Acid Quantification in Callus Culture of Satureja khuzistanica Jamzad (Lamiaceae). Iranian Journal of Pharmaceutical Research, 13(4), 1447-1456.
Amir Sahraroo; Mesbah Babalar; Mohammad Hossein Mirjalili; Mohammad Reza Fattahi Moghadam; Samad Nejad Ebrahimi. "In vitro Callus Induction and Rosmarinic Acid Quantification in Callus Culture of Satureja khuzistanica Jamzad (Lamiaceae)". Iranian Journal of Pharmaceutical Research, 13, 4, 2014, 1447-1456.
Sahraroo, A., Babalar, M., Mirjalili, M., Fattahi Moghadam, M., Nejad Ebrahimi, S. (2014). 'In vitro Callus Induction and Rosmarinic Acid Quantification in Callus Culture of Satureja khuzistanica Jamzad (Lamiaceae)', Iranian Journal of Pharmaceutical Research, 13(4), pp. 1447-1456.
Sahraroo, A., Babalar, M., Mirjalili, M., Fattahi Moghadam, M., Nejad Ebrahimi, S. In vitro Callus Induction and Rosmarinic Acid Quantification in Callus Culture of Satureja khuzistanica Jamzad (Lamiaceae). Iranian Journal of Pharmaceutical Research, 2014; 13(4): 1447-1456.

In vitro Callus Induction and Rosmarinic Acid Quantification in Callus Culture of Satureja khuzistanica Jamzad (Lamiaceae)

Article 39, Volume 13, Issue 4, Autumn 2014, Page 1447-1456  XML PDF (872 K)
Document Type: Research article
Authors
Amir Sahraroo1; Mesbah Babalar1; Mohammad Hossein Mirjalili 2; Mohammad Reza Fattahi Moghadam1; Samad Nejad Ebrahimi3
1Department of Horticulture, Faculty of Agriculture, University of Tehran, Karaj, Iran
2Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G. C., Evin, Tehran, Iran
3Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G. C., Evin, Tehran, Iran
Abstract
In the present study, an efficient protocol has been developed for callus induction and production of RA in callus culture of Satureja khuzistanica for the first time. In vitro callus induction was achieved from young shoot tip explants cultured on MS and B5 media supplemented with different concentrations of IBA (0.1, 1.0, 2.0 and 5.0 mgl-1) solely or in combination with cytokinins BAP and KIN (1.0, 2.0 and 5.0 mg l-1). B5 medium supplemented with 1.0 mgl-1 IBA plus 5.0 mgl-1 BAP and MS medium fortified with 2.0 mgl-1 IBA and 2.0 mgl-1 BAP were the most favorable media for callus formation with the highest induction rate (96%). Maximum growth index (2.89 and 2.63) and maximum callus biomass (2.34 and 2.33 g fresh weight) were obtained from the callus cultured on B5 medium supplemented with 1.0 mgl-1 IBA plus 5.0 mgl-1 BAP and MS medium fortified with 1.0 mgl-1 IBA plus 1.0 mgl-1 KIN, respectively. Determination and quantification of RA in cultured calli were performed by HPLC UV/MS analysis. Calli induced from the plant and maintained on supplements of IBA and BAP in the absence of light produced RA 7.5% based on dry weight (DW). No differentiation was observed in any callus during the course of this study.
Keywords
Satureja sp; Lamiaceae; Callus culture; Rosmarinic acid; HPLC UV/MS
Statistics
Article View: 7,485
PDF Download: 24,707
Home | Glossary | News | Aims and Scope | Sitemap
Top Top

Statistics

Journal Management System. Designed by sinaweb.