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Asadpour, E., Sadeghnia, H., Ghorbani, A., Boroushaki, M. (2014). Effect of Zirconium Dioxide Nanoparticles on Glutathione Peroxidase Enzyme in PC12 and N2a Cell Lines. Iranian Journal of Pharmaceutical Research, 13(4), 1141-1148. doi: 10.22037/ijpr.2014.1569
Elham Asadpour; Hamid Sadeghnia; Ahmad Ghorbani; Mohammad Taher Boroushaki. "Effect of Zirconium Dioxide Nanoparticles on Glutathione Peroxidase Enzyme in PC12 and N2a Cell Lines". Iranian Journal of Pharmaceutical Research, 13, 4, 2014, 1141-1148. doi: 10.22037/ijpr.2014.1569
Asadpour, E., Sadeghnia, H., Ghorbani, A., Boroushaki, M. (2014). 'Effect of Zirconium Dioxide Nanoparticles on Glutathione Peroxidase Enzyme in PC12 and N2a Cell Lines', Iranian Journal of Pharmaceutical Research, 13(4), pp. 1141-1148. doi: 10.22037/ijpr.2014.1569
Asadpour, E., Sadeghnia, H., Ghorbani, A., Boroushaki, M. Effect of Zirconium Dioxide Nanoparticles on Glutathione Peroxidase Enzyme in PC12 and N2a Cell Lines. Iranian Journal of Pharmaceutical Research, 2014; 13(4): 1141-1148. doi: 10.22037/ijpr.2014.1569

Effect of Zirconium Dioxide Nanoparticles on Glutathione Peroxidase Enzyme in PC12 and N2a Cell Lines

Article 4, Volume 13, Issue 4, Autumn 2014, Page 1141-1148  XML PDF (909.8 K)
Document Type: Research article
DOI: 10.22037/ijpr.2014.1569
Authors
Elham Asadpour1; Hamid Sadeghnia2; Ahmad Ghorbani3; Mohammad Taher Boroushaki email 4
1Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IRAN
2Department of Pharmacology and Neurocognitive Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IRAN
3Pharmacological Research Center of Medicinal Plants, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IRAN
4Pharmacological Research Center of Medicinal Plants and Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IRAN
Abstract
Objective:
Today, special attention is paid to the use of zirconium dioxide nanoparticle (nano-ZrO2), a neutral bioceramic metal, particularly for drug and gene delivery in medicine. However, there are some reports implying that use of nano-ZrO2 is associated with cytotoxic effects like inhibiting the cell proliferation, DNA damage and apoptosis. In the present study, we examined whether nano-ZrO2 alters cell viability and glutathione peroxidase (GPx) activity in two neuronal cell lines.
Materials and Methods:
The PC12 and N2a cells were cultured in the absence or presence of varying concentrations (31.25-2000 µg/ml) of nano-ZrO2 for 12, 24 or 48 h. The cell viability was evaluated using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay and GPx activity was determined by quantifying the rate of oxidation of the reduced glutathione to the oxidized glutathione.
Results:
Nano-ZrO2 caused a significant reduction in cell viability and GPx activity after 12, 24 and 48h, as compared with control group. These effects were concentration dependent and started from 250µg/ml.
Conclusion: The present study demonstrated that nano-ZrO2, at concentrations of > 250 µg/ml, has antiproliferative effects via reducing the cell defense mechanism against oxidative stress.
Keywords
Zirconium dioxide nanoparticles; PC12 cell line; N2a cell line; Oxidative Stress; Glutathion peroxidase enzyme
Main Subjects
Pharmacy; toxicology and Pharmacology
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