Khosravi zanjani, M., Ghiassi Tarzi, B., Sharifan, A., Mohammadi, N. (2014). Microencapsulation of probiotics by calcium alginate-gelatinized starch with chitosan coating and evaluation of survival in simulated human gastro-intestinal condition. Iranian Journal of Pharmaceutical Research, 13(3), 843-852. doi: 10.22037/ijpr.2014.1550
Mohammad ali Khosravi zanjani; Babak Ghiassi Tarzi; Anousheh Sharifan; Nima Mohammadi. "Microencapsulation of probiotics by calcium alginate-gelatinized starch with chitosan coating and evaluation of survival in simulated human gastro-intestinal condition". Iranian Journal of Pharmaceutical Research, 13, 3, 2014, 843-852. doi: 10.22037/ijpr.2014.1550
Khosravi zanjani, M., Ghiassi Tarzi, B., Sharifan, A., Mohammadi, N. (2014). 'Microencapsulation of probiotics by calcium alginate-gelatinized starch with chitosan coating and evaluation of survival in simulated human gastro-intestinal condition', Iranian Journal of Pharmaceutical Research, 13(3), pp. 843-852. doi: 10.22037/ijpr.2014.1550
Khosravi zanjani, M., Ghiassi Tarzi, B., Sharifan, A., Mohammadi, N. Microencapsulation of probiotics by calcium alginate-gelatinized starch with chitosan coating and evaluation of survival in simulated human gastro-intestinal condition. Iranian Journal of Pharmaceutical Research, 2014; 13(3): 843-852. doi: 10.22037/ijpr.2014.1550
Microencapsulation of probiotics by calcium alginate-gelatinized starch with chitosan coating and evaluation of survival in simulated human gastro-intestinal condition
1College of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, IranTehran, Iran.
2College of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3Department of Food Science and Technology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran
Abstract
Microencapsulation as one of the most modern methods has remarkable effects on probiotic survival. In this study Lactobacillus casei (ATCC 39392) and Bifidobacterium bifidum (ATCC 29521) were encapsulated using calcium alginate-gelatinized starch, chitosan coating and inulin via emulsion technique, and were incubated in simulated gastric juice (along with pepsin, pH=1.5) and simulated intestinal juice (along with pancreatin and bile salts, pH=8) for 2 hours at 37 oC. The morphology and size of microcapsules were measured by scanning electron and optical microscopy. The results indicated that the survival of microencapsulated probiotic increased significantly in simulated gastro-intestinal condition (P<0.05). Chitosan coating played a significant role in the protection of probiotic bacteria in simulated gastro-intestinal condition and the diameter of the microcapsules increased with the addition of chitosan coating. In general, this study indicated that microencapsulation with alginate-gelatinized starch coated with chitosan could successfully and significantly protect probiotic bacteria against adverse condition of simulated human gastro-intestinal condition.
Top