Document Type: Research article
Biopharmaceutics and Pharmacokinetic Division, Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Department of Veterinary Clinics, Faculty of Veterinary Medicine, University of Pisa, Via Livornese (lato monte) 1, San Piero a Grado 56010 Pisa, Italy
Chemical and Petroleum Engineering Department, Sharif University of Technology.
Tehran U of Medical Sciences
A rapid and sensitive HPLC method has been developed for the quantification of mirtazapine (MRZ), a noradrenergic and specific serotonergic inhibitor antidepressant (NaSSA) and its two major metabolites N-desmethylmirtazapine (NDM) and 8-hydroxymirtazapine (8-OHM) in human plasma.
The separation was achieved using Chromolith C18 column and a mobile phase of acetonitrile: phosphate buffer (pH=3, 20:80, v/v) in isocratic mode at a flow rate of 2 ml/min. A fluorescence detector was set at 290 and 350 nm for excitation and emission, respectively. Zolpidem was used as the internal standard. Liquid-liquid extraction was applied for sample clean up. All analytes were eluted in less than 5 minutes with LOQ of 1 ng/ml for MRZ and 2 ng/ml for both NDM and 8-OHM. The developed method was successfully applied to quantify MRZ and its metabolites in plasma of a healthy volunteer.