A rapid and sensitive HPLC-Fluorescence method for determination of Mirtazapine and its two major metabolites in human plasma

Document Type : Research article


1 Biopharmaceutics and Pharmacokinetic Division, Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

2 Department of Veterinary Clinics, Faculty of Veterinary Medicine, University of Pisa, Via Livornese (lato monte) 1, San Piero a Grado 56010 Pisa, Italy

3 Chemical and Petroleum Engineering Department, Sharif University of Technology.

4 Tehran U of Medical Sciences


A rapid and sensitive HPLC method has been developed for the quantification of mirtazapine (MRZ), a noradrenergic and specific serotonergic inhibitor antidepressant (NaSSA) and its two major metabolites N-desmethylmirtazapine (NDM) and 8-hydroxymirtazapine (8-OHM) in human plasma.
The separation was achieved using Chromolith C18 column and a mobile phase of acetonitrile: phosphate buffer (pH=3, 20:80, v/v) in isocratic mode at a flow rate of 2 ml/min. A fluorescence detector was set at 290 and 350 nm for excitation and emission, respectively. Zolpidem was used as the internal standard. Liquid-liquid extraction was applied for sample clean up. All analytes were eluted in less than 5 minutes with LOQ of 1 ng/ml for MRZ and 2 ng/ml for both NDM and 8-OHM. The developed method was successfully applied to quantify MRZ and its metabolites in plasma of a healthy volunteer.


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