Effect of Dexamethasone, Insulin and EGF on the Myogenic Potential on Human Endometrial Stem Cell

Document Type: Research article

Authors

1 Depatment of Biology, Science and Research Branch Islamic Azad University Tehran Iran.

2 Department of Tissue Engineering, Faculty of Advance Technology, University of Medical Sciences, Tehran, Iran.

3 Paediatric Urology Research Center, Department of Urology, Children's Hospital Medical Center, Tehran University of Medical Sciences,Tehran, Iran

4 Umbilical Cord Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

5 Associate of prod.,Departmaent of Genetics,IAU,Tehran medical branch

6 Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.

Abstract

Human endometrium contains mesenchymal stem cells (eMSC) which have the ability to differentiate into three cell lineages and the potential in therapeutic applications. We hypothesize that using environmental induction in culture media such as dexamethasone, human recombinant insulin and human epidermal growth factor (hEGF) can differentiate endometrial stem cells into myoblast. These agents have a broad range of effects in myoblast differentiation in vitro. We used immunohystochemistry analysis and RT –PCR to evaluate the presence of skeletal muscle - specific proteins some of which are expressed in the early stage of differentiation including myoD and Desmin which expressed at later stages of differentiation. In conclusion eMSC can differentiate in culture media which contains above mentioned factors and use for therapeutic purpose in muscular degenerative disease.

Keywords