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Fan, P., Ren, J., Ma, H., Jing, L., Ma, J., Jia, Z. (2014). Liquid Chromatography Tandem Mass/Mass Spectrometry for the Quantification of Fudosteine in Human Serum without Precolumn Derivatization. Iranian Journal of Pharmaceutical Research, 13(2), 441-447. doi: 10.22037/ijpr.2014.1490
Peng-Cheng Fan; Jun Ren; Hui-Ping Ma; Lin-Lin Jing; Jun Ma; Zheng-Ping Jia. "Liquid Chromatography Tandem Mass/Mass Spectrometry for the Quantification of Fudosteine in Human Serum without Precolumn Derivatization". Iranian Journal of Pharmaceutical Research, 13, 2, 2014, 441-447. doi: 10.22037/ijpr.2014.1490
Fan, P., Ren, J., Ma, H., Jing, L., Ma, J., Jia, Z. (2014). 'Liquid Chromatography Tandem Mass/Mass Spectrometry for the Quantification of Fudosteine in Human Serum without Precolumn Derivatization', Iranian Journal of Pharmaceutical Research, 13(2), pp. 441-447. doi: 10.22037/ijpr.2014.1490
Fan, P., Ren, J., Ma, H., Jing, L., Ma, J., Jia, Z. Liquid Chromatography Tandem Mass/Mass Spectrometry for the Quantification of Fudosteine in Human Serum without Precolumn Derivatization. Iranian Journal of Pharmaceutical Research, 2014; 13(2): 441-447. doi: 10.22037/ijpr.2014.1490

Liquid Chromatography Tandem Mass/Mass Spectrometry for the Quantification of Fudosteine in Human Serum without Precolumn Derivatization

Article 10, Volume 13, Issue 2, Spring 2014, Page 441-447  XML PDF (1008.41 K)
Document Type: Research article
DOI: 10.22037/ijpr.2014.1490
Authors
Peng-Cheng Fan; Jun Ren; Hui-Ping Ma; Lin-Lin Jing; Jun Ma; Zheng-Ping Jia email
General Hospital of Lanzhou command of PLA
Abstract
A quantitative analysis method for fudosteine in human serum by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI/MS/MS) was established, which shows high sensitivity and selectivity. The mobile phase composition was 75% 20 mM acetic acid and 25% acetonitril, which was pumped at a flow rate of 0.40 mL/min. The overall chromatographic run time was approximately 7 min. The autosampler was set with an injection volume of 10 μl. The calibration curve was linear in the concentration range of 0.1~15.0 µg/mL. The coefficient of determination (r) was greater than 0.9998. This method has been fully validated and shown to be specific, accurate and precise. The method was simple, rapid and the sample preparation was minimal. It was successfully applied to the analysis of healthy volunteer serum samples during a phaseⅠclinical pharmacokinetic study of fudosteine.
Keywords
Fudosteine; HPLC-ESI/MS/MS; Human serum; Pharmacokinetics
Main Subjects
Drug quality control; Pharmacutics
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