Interactive effect of two treatment methods on the reduction of withdrawal syndrome signs in male rats



Drug addiction is a destructive misfortune that leads to recession in all social fields. For this reason, proper treatment of addiction should be seriously considered. Formerly, the effectiveness of TENS and ICWS methods on the reduction of withdrawal syndrome signs in the rat was evaluated separately. In this study the interaction of these two methods were analyzed. For this purpose, four groups of rats (Spruge-Dawley) made dependent using injection of morphine (s.c.). Then, rats were exposed to high frequency TENS and ICWS, alone or in combination. In all groups, naloxone was injected (i.p.) to induce withdrawal syndrome. Statistical analysis was performed using ANOVA, Kruskal-Wallis, and Mann-Witney. The results showed that TENS method was very effective to reduce jumping and ptosis. In contrast, using ICWS a significant reduction in diarrhea, weight loss, yawning and abnormal posture was observed. Using both methods, only few signs were reduced. It is recommended to use these methods in clinical trials.