Effect of Adiantum Capillus veneris Linn on an Animal Model of Testosterone-Induced Hair Loss

Document Type: Research article

Authors

1 Department of Pharmacology and Toxicology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran

2 School of Pharmacy, Shahid Beheshti University of Medical Science

3 Department of Pharmaceutics, School of Pharmacy, Shahid Beheshti University of Medical Sciences

4 Department of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences

5 Department of Pharmacology and Toxicology, School of Pharmacy, Zanjan University of Medical Sciences

6 Department of Clinical Pharmacy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Abstract

Androgenetic alopecia is the most common form of hair loss in men. The present study was designed to evaluate the hair growth-promoting activity of a preparation of the Adiantum capillus veneris Linn. (A. capillus veneris Linn.) on albino mice using a testosterone-induced alopecia model. Five groups of albino mice were studied: (A) Testosterone solution only (n=6); (B) Testosterone ‏+ Finasteride solution (2%) (n=6); (C) Testosterone ‏+ vehicle (n=6); (D) Testosterone + A. capillus veneris Linn. solution (1%) (n=6); (E) intact control (n=2, without testosterone). Alopecia was induced in all intervention groups by testosterone 1.0 mg subcutaneous. A. capillus veneris Linn. solution was applied topically to the back skin of animals in the respective group. Hair growth was evaluated by visual observation and histological study of several skin sections via various parameters as follicle density (number of follicles/mm) and anagen/telogen ratio. After 21 days, a patch of diffuse hair loss was seen in animals received testosterone while animals treated with A. capillus veneris Linn. showed less hair loss as compared to those treated with testosterone only. The follicular density observed in the A. capillus veneris Linn.-treated group was 1.92±0.47, compared to 1.05±0.21 in testosterone-group and 2.05±0.49 in finasteride-treated animals. Anagen/telogen ratio was significantly affected by A. capillus veneris Linn., which was 0.92±0.06 as compared with 0.23±0.03 and 1.12±0.06 for testosterone and finasteride treated groups, respectively. According to visual observation and quantitative data (follicular density and anagen/telogen ratio), A. capillus veneris Linn. was found to possess good activity against testosterone-induced alopecia.

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