The Study of Petoxifylline Drug Effects on Renal Apoptosis and Bcl2 Gene Expression Changes Following Ischemic Reperfusion Injury in Rat

Document Type : Research article


Associate of prod.,Departmaent of Genetics,IAU,Tehran medical branch


Background & Target: Ischemia Reperfusion injury is the tissue damage caused when blood supply returns to the tissue after a period of ischemia or lack of oxygen. In this study, the effect of pentoxyfylline on bcl2 gene expression changes and cell injury in kidney of rat following Ischemia Reperfusion were evaluated.
Methods: In this experimental study, 20 male wistar rats with average weight of 250-300g were selected and then were accidently divided them on two tenth group of control and treatment groups. In the control group, celiotomy was performed by ventral midline incision. The left kidney was isolated, and then both the renal artery and vein were obstructed. After 60 minutes of warm ischemia, vessel obstruction resolved and the right kidney was removed. 72 hours after reperfusion, tissue samples were taken from left kidney for Tunel assay. We used quantitative real time PCR for detection of bcl2 gene expression in treated groups and then compared them to control samples.
Results: In the treatment group, the cell death changes, showed lower level than the control group. The results also showed the bcl2 gene expression was declined in ischemia group as campared to PNT drug group.
Conclusion: The pentoxyfylline might have a role in control of apoptosis result from Ischemia- reperfusion and quantitative real-time PCR can be used as a direct method for detection bcl2 gene expression in tested samples and normal samples