Toxicity Effect of Silver Nanoparticles on Mice Liver Primary Cell Culture and HepG2 Cell Line

Document Type: Research article


1 Faculty of Veterinary Medicine, Islamic Azad University - Science and Research Branch, Tehran, Iran

2 Pharmaceutical sciences research center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3 Deptartment of Toxico-Pharmacology, Faculty of pharmacy, Shahid Beheshti Medical University, Tehran, Iran

4 Department of Biochemistry and Nutrition, Zanjan University of Medical Sciences, Iran Islamic Azad University, Pharmaceutical seines Branch, Tehran, Iran.

5 Department of Basic Science, Science and Research branch, Islamic Azad University, Tehran, Iran.


Nano-silver (AgNP) has biological properties which are significant for consumer products, food technology, textiles and medical applications (e.g. wound care products, implantable medical devices, in diagnosis, drug delivery, and imaging).
For their antibacterial activity, silver nanoparticles are largely used in various commercially available products. Thus, the use of nano-silver is becoming more and more widespread in medicine.
In this study we investigated the cytotoxic effects of AgNPs on liver primary cells of mice, as well as the human liver HepG2 cell.
Cell viability was examined with MTT assay after HepG2 cells exposure to AgNPs at 1, 2, 3, 4, 5, 7.5, 10 ppm compared to mice primary liver cells at 1, 10, 50, 100, 150, 200, 400 ppm for 24h. AgNPs caused a concentration-dependent decrease of cell viability in both cells. IC50 value of 2.764 ppm (µg/ml) was calculated in HepG2 cell line and IC50 value of 121.7 ppm (µg/ml) was calculated in primary liver cells of mice. The results of this experiment indicated that silver nanoparticles had cytotoxic effects on HepG2 cell line and primary liver cells of mice. The results illustrated that nano-silver had 44 times stronger inhibitory effect on the growth of cancerous cells (HepG2 cell line ) compared to the normal cells (primary liver cells of mice). which might further justify AgNPs as a cytotoxic agents and a potential anticancer candidate which needs further studies in this regard.


Main Subjects