Pourahmad Jaktaji, R., Ebadi, R. (2013). Study the Expression of marA Gene in Ciprofloxacin and
Tetracycline Resistant Mutants of Esherichia coli. Iranian Journal of Pharmaceutical Research, 12(4), 923-928. doi: 10.22037/ijpr.2013.1408
Razieh Pourahmad Jaktaji; Rayhaneh Ebadi. "Study the Expression of marA Gene in Ciprofloxacin and
Tetracycline Resistant Mutants of Esherichia coli". Iranian Journal of Pharmaceutical Research, 12, 4, 2013, 923-928. doi: 10.22037/ijpr.2013.1408
Pourahmad Jaktaji, R., Ebadi, R. (2013). 'Study the Expression of marA Gene in Ciprofloxacin and
Tetracycline Resistant Mutants of Esherichia coli', Iranian Journal of Pharmaceutical Research, 12(4), pp. 923-928. doi: 10.22037/ijpr.2013.1408
Pourahmad Jaktaji, R., Ebadi, R. Study the Expression of marA Gene in Ciprofloxacin and
Tetracycline Resistant Mutants of Esherichia coli. Iranian Journal of Pharmaceutical Research, 2013; 12(4): 923-928. doi: 10.22037/ijpr.2013.1408
Study the Expression of marA Gene in Ciprofloxacin and
Tetracycline Resistant Mutants of Esherichia coli
1Department of Genetics, Faculty of Science, University of Shahrekord and Biotechnology Center, University of Shahrekord, Shahrekord, Iran.
2Genetics at University of Shahrekord.
Abstract
MarA activates two membrane dependent mechanisms of resistance to different antibiotics, such as ciprofloxacin and tetracycline, including promotion of outflux and inhibition of influx of antibiotics. Thus, MarA causes multiple antibiotic resistance phenotype. The activation of these mechanisms needs overexpression of marA. This could happen through mutation in marR. Thus, the aim of this study was to measure marA expression in ciprofloxacin resistant E. coli gyrA mutants and clones with or without marR mutation. For this purpose, real time PCR was used to measure relative expression of marA in above mutants and clones. Results showed that two clones, C14 and C17 overexpressed marA. It is concluded that the level of marA expression is important for activation of above mechanisms.
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