Quantitative determination of three angiotensin-II-receptor antagonists in presence of Hydrochlorothiazide by RP-HPLC in their tablets preparations

Document Type: Research article

Authors

Medicinal Chemistry Department Faculty of Pharmacy, Zagazig University, Zagazig, Egypt. Chemistry, Nancy University, France, QC Manager, EIPICO, 10th Ramadan, Egypt.

Abstract

Losartan potassium, Valsartan , Telmisartan and Irbesartan are angiotensin-II-receptor antagonists (ARA II) group which used in treatment of hypertension alone or in combination with other drugs mainly Hydrochlorothiazide. RP- HPLC method was developed for the assay of three angiotensin-II-receptor antagonists (ARA-IIs) in presence of Hydrochlorothiazide. The method was performed by reversed phase high performance liquid chromatography using a mobile phase which is consisted of 0.025 M potassium dihydrogen phosphate (pH 6.0): acetonitrile =65:35% with detection at 220 nm on an ACE C18 column (250 mm × 4.6 mm, 5 μm) at flow rate 1.5 ml/min in an isocratic manner. The proposed method was validated according to ICH guidline in terms of linearity, accuracy, precision , robustness, limit of detection and limit of quantitation.

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