Suppressive Effects of Rosa Damascena Essential Oil on Naloxone-Precipitated Morphine Withdrawal Signs in Male Mice

Document Type: Research article


1 Departement of Pharmacology, Urmia Branch, Islamic Azad University, Urmia, Iran.

2 Research Development and Training center, Barij Essence Pharmaceutical CO, Kashan, Iran.


This research was done to test the effect of Rosa damascena essential oil on withdrawal signs
of naloxone-precipitated morphine in male mice. Morphine dependence was induced by injection
(IP) three times daily at doses of 50, 50 and 75 mg/kg, respectively, for 3 days. On day 4,
after the last administration of morphine, Rosa damascena essential oil was administered at
different concentrations (5, 2 and 40%, IP) 30 min before administration of naloxone (5 mg/kg,
IP). The following actions were taken as signs of withdrawal and records taken for jumping as
a number and scores of 0 to 3 were given for incidences of grooming, teeth chattering, rearing,
writing, diarrhea, wet dog shakes and climbing during a 30 min period. Results showed that
different concentrations of Rosa damascena essential oil significantly reduced signs of morphine
withdrawal compared to the control group in terms of number of jumps (p < 0.05 and p < 0.01),
grooming, teeth chattering, rearing, climbing, wet dog shakes and writhing, but not for diarrhea
(p < 0.05). In conclusion it seems that GABAergic activity induced by flavonoids from Rosa
damascena essential oil can alleviate signs of morphine withdrawal, but further studies need to be
done to better understand this mechanism.