Document Type: Research article
Dental Implant Research Center and Prosthodontics, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, IR Iran.
Dental materials research center and Prosthodontics, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, IR Iran.
Biomaterials Research Group, Department of Materials Engineering, Isfahan University of Technology, Isfahan, IR Iran.
Department of Medicinal Chemistry, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, IR Iran.
The cytotoxicity of the biomaterials is a key issue that should be addressed prior to pre-clinical applications. This study was designed to evaluate and compare the cytotoxixity of two forms of bioactive glasses:nanopowder:andmicropowder. Human HGF1-PI53 gingival fibroblast cells were used to evaluate the cytotoxicity of 0.5, 1, 1.5, 2, 5, 10, 15 and 20 mg/mL concentrations of the two bioactive glasses via MTT assay. The results were statistically analyzed using analysis of variance and Tukey's test. A p-value less than 0.05 was considered statistically significant. Results showed that two bioactive glasses hadstatistically significant differences at 5, 10, 15 and 20 mg/mL concentrations (p-value < 0.05) and there was no correlation between time and cell cytotoxicity of bioactive glasses (p-value > 0.05), using t-test and Spearman’s correlation coefficient. We conclude that that cytotoxicity of nanopowder bioactive glass atconcentrations ≤ 2 mg/mL was similar to micropowder bioactive glass at 24 and 48 h, however, it is more cytotoxic atconcentrations ≥ 5 mg/mL in the first 48 h of applications.