Kazemipour, M., Fakhari, I., Ansari, M. (2013). Gabapentin Determination in Human Plasma and Capsule by Coupling of Solid Phase Extraction, Derivatization Reaction, and UV-Vis Spectrophotometry. Iranian Journal of Pharmaceutical Research, 12(3), 247-253. doi: 10.22037/ijpr.2013.1347
Maryam Kazemipour; Iman Fakhari; Mehdi Ansari. "Gabapentin Determination in Human Plasma and Capsule by Coupling of Solid Phase Extraction, Derivatization Reaction, and UV-Vis Spectrophotometry". Iranian Journal of Pharmaceutical Research, 12, 3, 2013, 247-253. doi: 10.22037/ijpr.2013.1347
Kazemipour, M., Fakhari, I., Ansari, M. (2013). 'Gabapentin Determination in Human Plasma and Capsule by Coupling of Solid Phase Extraction, Derivatization Reaction, and UV-Vis Spectrophotometry', Iranian Journal of Pharmaceutical Research, 12(3), pp. 247-253. doi: 10.22037/ijpr.2013.1347
Kazemipour, M., Fakhari, I., Ansari, M. Gabapentin Determination in Human Plasma and Capsule by Coupling of Solid Phase Extraction, Derivatization Reaction, and UV-Vis Spectrophotometry. Iranian Journal of Pharmaceutical Research, 2013; 12(3): 247-253. doi: 10.22037/ijpr.2013.1347
Gabapentin Determination in Human Plasma and Capsule by Coupling of Solid Phase Extraction, Derivatization Reaction, and UV-Vis Spectrophotometry
1Department of Chemistry, Faculty of Sciences, Kerman Branch, Islamic Azad University, IR Iran.
2Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Abstract
Gabapentin is an anticonvulsant widely used in the treatment of epilepsy. No peculiar chromophore is available on the gabapentin moiety for direct analysis by absorption spectrophotometry. A sensitive spectrophotometric method for the determination of gabapentin in bulk, pharmaceutical formulations and human plasma has been developed. In this method, gabapentin directly derivatized with vanillin and analyzed without any extraction in bulk and pharmaceutical dosage form and in plasma samples, it was extracted with a reversed-phase solid-phase extraction (SPE) cartridge followed by derivatization with vanillin. Analysis was performed by a spectrophotometer system. The quantitation limit of gabapentin in human plasma was 0.8 mg/L. The method was linear over the concentration range of 10.0–90.0 mg/L and 0.8–10.0 mg/L for pharmaceutical dosage form and plasma, respectively. The method was precise (relative standard deviation, RSD <1.20%) and accurate (relative mean error <5.5%) for both pharmaceutical dosage form and plasma samples. Mean absolute recoveries were 94.5% for plasma.
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