The Effect of Vitis vinifera L. Leaves Extract on Leishmania infantum In-vitro. 

Document Type : Research article


1 Unité of Recherche 12-04, Chimie Appliquée and Environnement, Faculté of Sciences of Monastir, Monastir 5000, Tunisie.

2 Laboratoire of Parasitologie-Mycologie (99UR/08-05), Faculté de Pharmacie, Département of Biologie, Clinique B, 1 Rue Avicenne, Monastir 5000, Tunisie.

3 Laboratoire of Biodiversité and Biotechnologie Marine, Institut National des Sciences and Technologies of la Mer, Annexe Monastir, BP 59, Monastir 5000, Tunisie.


Vitis vinifera a traditional Asian herb widely used for different health problems. In the
present research, the ethanolic and the aqueous extracts of Vitis vinifera L. leaves collected from
shrub, grown in Tunisia, were prepared and evaluated for the antileishmanial activity against
Leishmani ainfantum promastigotes. The inhibitory concentration 50 (IC50) was determined and
the results showed that the etahnolic extract is more active than the aqueous one (IC50= 0.108
mg/mL). Microscopic observations showed that the ethanolic extract promoted the destruction
of cytoplasmic and nuclear membranes of Leishmani ainfantum promastigotes and altered the
overall shape of the cell. In order to explain the difference of antileishmanial activity between
ethanolic and aqueous extracts, anthocyanins amount was determined by spectrophotometry. It
was found that the ethanolic extract is richer in anthocyanins than the aqueous one which can
explain the higher antileishmanial activity of the ethanolic extract.