Effect of Laurus nobilis L. Essential Oil and its Main Components on α-glucosidase and Reactive Oxygen Species Scavenging Activity

Document Type: Research article


Department of Biochemistry, Faculty of Sciences, Cumhuriyet University, 58140, Sivas, Turkey.


The present study was designed to determine the effects of the essential oil of Laurus
nobilis L. (Lauraceae) and its three main components on α-glucosidase and reactive oxygen
species scavenging activity. The chemical composition of the essential oil from Laurus nobilis
L. leaves was analyzed by GC/GC-MS and resulted in the identification of 29 compounds,
representing 99.18% of the total oil. 1,8-cineole (68.82%), 1-(S)-α-pinene (6.94%), and R-(+)-
limonene (3.04%) were determined to be the main components. The antioxidant features of the
essential oil and its three main components were evaluated using inhibition of 2,2-diphenyl-1-
picrylhydrazyl, hydroxyl, and superoxide radicals, inhibition of hydrogen peroxide and lipid
peroxidation assays. The results show that the DPPH, hydroxyl, and superoxide radical as well
as hydrogen peroxide scavenging activities of the essential oil are greater than the positive
controls and the three main components of the oil when tested independently. The inhibition
of lipid peroxidation by the oil occurred less frequently than with 1,8-cineole and R-(+)-
limonene alone, but the effects were more pronounced than those seen with 1-(S)-α-pinene
and the positive controls. An α-glucosidase inhibition assay was applied to evaluate the in-vitro
antidiabetic activity of the essential oil. IC50-values were obtained for laurel essential oil, 1,
8-cineole, 1-(S)-α-pinene, and R-(+)-limonene: 1.748 μL/mL, 1.118 μL/mL, 1.420 μL/mL and
1.300 μL/mL, respectively. We also found that laurel essential oil and 1, 8-cineole inhibited
the α-glucosidase competitively while 1-(S)-α-pinene and R-(+)-limonene were uncompetitive


Main Subjects