Souri, E., Hatami, A., Shabani Ravari, N., Alvandifar, F., Barazandeh Tehrani, M. (2013). Validating a Stability Indicating HPLC Method for Kinetic Study of
Cetirizine Degradation in Acidic and Oxidative Conditions. Iranian Journal of Pharmaceutical Research, 12(2), 287-294.
Effat Souri; Ali Hatami; Nazanin Shabani Ravari; Farhad Alvandifar; Maliheh Barazandeh Tehrani. "Validating a Stability Indicating HPLC Method for Kinetic Study of
Cetirizine Degradation in Acidic and Oxidative Conditions". Iranian Journal of Pharmaceutical Research, 12, 2, 2013, 287-294.
Souri, E., Hatami, A., Shabani Ravari, N., Alvandifar, F., Barazandeh Tehrani, M. (2013). 'Validating a Stability Indicating HPLC Method for Kinetic Study of
Cetirizine Degradation in Acidic and Oxidative Conditions', Iranian Journal of Pharmaceutical Research, 12(2), pp. 287-294.
Souri, E., Hatami, A., Shabani Ravari, N., Alvandifar, F., Barazandeh Tehrani, M. Validating a Stability Indicating HPLC Method for Kinetic Study of
Cetirizine Degradation in Acidic and Oxidative Conditions. Iranian Journal of Pharmaceutical Research, 2013; 12(2): 287-294.
Validating a Stability Indicating HPLC Method for Kinetic Study of
Cetirizine Degradation in Acidic and Oxidative Conditions
Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Abstract
A stability indicating High-Performance Liquid Chromatography (HPLC) method was validated and used to study the degradation of cetirizine dihydrochloride in acidic and oxidative conditions. The separation was carried out on a Symmetry C18 column and a mixture of 50 mM KH2PO4 and acetonitrile (60:40 v/v, pH = 3.5) was used as the mobile phase. The method was linear over the range of 1-20 μg/mL of cetirizine dihydrochloride (r2 > 0.999) and the withinday and between-day precision values were less than 1.5%. The results showed that cetirizine dihydrochloride was unstable in 2 M HCl and 0.5% H2O2. The kinetics of the acidic degradation showed a pseudo-first-order reaction in the temperature range of 70-90°C. In addition, the kinetics of hydrogen peroxide mediated degradation was pseudo-first-order in the temperature range of 50-80°C.
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