Kwon, J., Jiang, H., Minai-Tehrani, A., Woo, C., Choi, M., Cho, C., Kim, Y., Cho, M. (2013). Gene Expression and Pulmonary Toxicity of Chitosan-graft-Polyethylenimine as Aerosol Gene Carrier. Iranian Journal of Pharmaceutical Research, 12(2), 281-286. doi: 10.22037/ijpr.2013.1311
Jung-Taek Kwon; Hu-Lin Jiang; Arash Minai-Tehrani; Chang Gyu Woo; Mansoo Choi; Chong-Su Cho; Yeon-Soo Kim; Myung-Haing Cho. "Gene Expression and Pulmonary Toxicity of Chitosan-graft-Polyethylenimine as Aerosol Gene Carrier". Iranian Journal of Pharmaceutical Research, 12, 2, 2013, 281-286. doi: 10.22037/ijpr.2013.1311
Kwon, J., Jiang, H., Minai-Tehrani, A., Woo, C., Choi, M., Cho, C., Kim, Y., Cho, M. (2013). 'Gene Expression and Pulmonary Toxicity of Chitosan-graft-Polyethylenimine as Aerosol Gene Carrier', Iranian Journal of Pharmaceutical Research, 12(2), pp. 281-286. doi: 10.22037/ijpr.2013.1311
Kwon, J., Jiang, H., Minai-Tehrani, A., Woo, C., Choi, M., Cho, C., Kim, Y., Cho, M. Gene Expression and Pulmonary Toxicity of Chitosan-graft-Polyethylenimine as Aerosol Gene Carrier. Iranian Journal of Pharmaceutical Research, 2013; 12(2): 281-286. doi: 10.22037/ijpr.2013.1311
Gene Expression and Pulmonary Toxicity of Chitosan-graft-Polyethylenimine as Aerosol Gene Carrier
1Laboratory of Toxicology, College of Veterinary Medicine, Seoul 151-742, Korea.
21- Laboratory of Toxicology, College of Veterinary Medicine, Seoul 151-742, Korea. 2- School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China.
3Nanotechnology and Thermal Processing Laboratory, School of Mechanical and Aerospace Engineering, Seoul 151-742, Korea.
4Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Korea.
5Indang Institute of Molecular Biology, Inje University, Seoul 100-032, Korea.
61- Laboratory of Toxicology, College of Veterinary Medicine, Seoul 151-742, Korea. 2- Department of Nanofusion Technology, Graduate School of Convergence Science and Technology, Seoul National University, Suwon 443-270, Korea. 3- Advanced Institute of Convergence Technology, Seoul National University, Suwon 443-270, Korea.
Abstract
Chitosan-graft-polyethylenimine (CHI-g-PEI) copolymer has been used for the improvement of low transfection efficiency of chitosan. The present study aims to test the pulmonary toxicity and efficiency of CHI-g-PEI as an aerosol gene carrier. Mice were exposed to aerosol containing green-fluorescent protein (GFP)-polyethylenimine (PEI) or GFP-CHIg- PEI complexes for 30 min during the development of our nose-only exposure chamber (NOEC) system. CHI-g-PEI-mediated aerosol delivery demonstrated 15.65% enhancement of the fluorescence intensity. Compared to PEI, CHI-g-PEI showed no significant pulmonary toxicity. In summary, using CHI-g-PEI is safe and shows high transfection in aerosol gene delivery to animals, and enhanced efficiency was achieved through our aerosol gene delivery system. Therefore, CHI-g-PEI and this system would be applicable to future study for aerosol gene therapy.
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