Maham, M., Kiarostami, V., Husain, S., Abroomand-Azar, P., Saber-Tehrani, M., Khoeini Sharifabadi, M., Afrouzi, H., Shapouri, M., Karami-Osboo, R. (2013). Extraction and Determination of Cyproheptadine in Human Urine by DLLME-HPLC Method. Iranian Journal of Pharmaceutical Research, 12(2), 311-318.
Mehdi Maham; Vahid Kiarostami; Syed Waqif Husain; Parviz Abroomand-Azar; Mohammad Saber-Tehrani; Malihe Khoeini Sharifabadi; Hossein Afrouzi; Mahmoudreza Shapouri; Rouhollah Karami-Osboo. "Extraction and Determination of Cyproheptadine in Human Urine by DLLME-HPLC Method". Iranian Journal of Pharmaceutical Research, 12, 2, 2013, 311-318.
Maham, M., Kiarostami, V., Husain, S., Abroomand-Azar, P., Saber-Tehrani, M., Khoeini Sharifabadi, M., Afrouzi, H., Shapouri, M., Karami-Osboo, R. (2013). 'Extraction and Determination of Cyproheptadine in Human Urine by DLLME-HPLC Method', Iranian Journal of Pharmaceutical Research, 12(2), pp. 311-318.
Maham, M., Kiarostami, V., Husain, S., Abroomand-Azar, P., Saber-Tehrani, M., Khoeini Sharifabadi, M., Afrouzi, H., Shapouri, M., Karami-Osboo, R. Extraction and Determination of Cyproheptadine in Human Urine by DLLME-HPLC Method. Iranian Journal of Pharmaceutical Research, 2013; 12(2): 311-318.
Extraction and Determination of Cyproheptadine in Human Urine by DLLME-HPLC Method
1Department of Chemistry, Science and Research Branch, Islamic Azad University ,P.O.Box 14515-775, Poonak Hesarak, Tehran, Iran.
2Department of Chemistry, North Tehran Branch, Islamic Azad University, Tehran, Iran.
3Quality Control laboratory, DarouPakhsh Mfg. Co., Tehran, Iran.
4Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.
Abstract
Novel dispersive liquid-liquid microextraction (DLLME), coupled with high performance liquid chromatography with photodiode array detection (HPLC-DAD) has been applied for the extraction and determination of cyproheptadine (CPH), an antihistamine, in human urine samples. In this method, 0.6 mL of acetonitrile (disperser solvent) containing 30 µL of carbon tetrachloride (extraction solvent) was rapidly injected by a syringe into 5 mL urine sample. After centrifugation, the sedimented phase containing enriched analyte was dissolved in acetonitrile and an aliquot of this solution injected into the HPLC system for analysis. Development of DLLME procedure includes optimization of some important parameters such as kind and volume of extraction and disperser solvent, pH and salt addition. The proposed method has good linearity in the range of 0.02-4.5 µg mL-1 and low detection limit (13.1 ng mL-1). The repeatability of the method, expressed as relative standard deviation was 4.9% (n = 3). This method has also been applied to the analysis of real urine samples with satisfactory relative recoveries in the range of 91.6-101.0%.
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