Increased activity of mesolimbic dopaminergic system within ventral tegmental area (VTA) due to nicotine results in psychologic dependence. It has been argued that function of dopamine receptors could by affected by ascorbic acid (AA). Furthermore, AA can decrease the withdrawal syndrome signs in addicted animals and may postpone the development of nicotine dependence. In the present study, the possible role of AA on acquisition and expression of nicotine-induced CPP was investigated in mice. Female Swiss-Webster mice (20-25 g) were used and a biased method was performed for induction of CPP. The results showed that i.p. injection of nicotine (0.25-2 mg/kg) induced CPP in a dose-dependent manner. In addition, IP administration of AA (1-1000 mg/kg) on the test day reduced the nicotine effect, but not in a dose-dependent manner. The chronic administration of AA (1-1000 mg/kg) reduced the nicotine effect as well. Therefore, it can be concluded that motivational effects of nicotine are dramatically affected by AA.