Primary Assessment of Mycosporine-Like Amino Acids Production by Two Species of Fischerella sp.

Document Type : Research article


1 Protein Technology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

2 Department of Pharmacognosy , School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

3 Department of Pharmacognosy and Pharmaceutical Biotechnology, Faculty of Pharmacy , Hormozgan University of Medical Sciences, Bandar Abbas, Iran.



Mycosporin-like amino acids (MAAs) are a group of UV-absorbing compounds, which can be produced by various organisms such as algae and cyanobacteria, particularly if they survive in highly irradiated environments. In this study, the production of MAAs by two species of Fischerlla sp. (F5 and F14), isolated from the North of Iran, was investigated. Both species, which had previously been morphologically detected as Fisherella sp., were confirmed molecularly by sequencing the PCR amplicon of the 16S rRNA gene. The species were cultured in sterilized BG.11 medium for 21 days, then biomasses were separated, and their MAAs content was extracted by methanol and partially purified using chloroform liquid-liquid extraction. The extract was analyzed using high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectroscopy (LC-MS). In both species, the compounds with MAAs characteristics were observed. They had maximum absorbance (λmax) in the range of 300–400 nm, which was confirmed by the LC-MS analysis. In F5 species, the peaks with m/z 340 and 391 and in another one (F14), a peak with m/z 333.2 were recorded, that the latter might be Shinorine. In general, further analysis should be performed to elucidate the exact structural aspects of these compounds. In conclusion, both Fischerella sp. studied here were capable of producing MAAs and can be evaluated for use in sunscreen pharmaceutical and cosmetic products.

Graphical Abstract

Primary Assessment of Mycosporine-Like Amino Acids Production by Two Species of Fischerella sp.


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