Healing Influence of Melilotus Offcinalis Herbal Extract on Experimental Autoimmune Encephalomyelitis in C57BL/6 Mice

Document Type : Research article


1 Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.

2 Department of Basic Sciences, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.

3 School of Medicine, AJA University of Medical Science, Tehran, Iran.


The present study was designed to primarily examine the therapeutic potential of the herbal extract of Melilotus Offcinalis for the treatment of multiple sclerosis in the experimental autoimmune encephalomyelitis (EAE) model of the disease. The animal model was induced in C57BL/6 female mice, and then the herbal extract was intraperitoneally administered for a total of 21 days after the frst day of post-immunization. The phenotypic signs, a gene expression profle of inflammatory cytokines, antioxidant state, and pathological hallmarks of the disease in the corpus callosum were evaluated. The prophylactic administration of Melilotus Offcinalis attenuates the clinical signs of the disease. It signifcantly declined the gene expression of proinflammatory cytokines like IL-6, TNF-α, and IFN-γ. This herbal extract also surged the gene expression, as an anti-inflammatory cytokine. The gene expression of Glutathione peroxidase and Catalase (antioxidant enzymes) was meaningfully higher in the treatment group. Pathological evaluation of corpus callosum cross-sections by Luxol Fast Blue staining revealed preserved myelin sheath in the treated group compared to the EAE mice. The results of our assay confrmed that immunomodulatory and antioxidant features of the herbal extract of Melilotus Offcinalis ameliorated the EAE  severity. This study fnding disclosed the therapeutic effciency of this compound in MS treatment.

Graphical Abstract

Healing Influence of Melilotus Offcinalis Herbal Extract on Experimental Autoimmune Encephalomyelitis in C57BL/6 Mice


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