Chitosan/PVA/Doxycycline Film and Nanofber Accelerate Diabetic Wound Healing in a Rat Model

Document Type : Research article


1 Department of Physiology and Pharmacology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran. Cardiovascular Research Center, Alborz University of Medical Sciences, Karaj, Iran.

2 Polymer and Color Engineering Department Amirkabir University of Technology (Tehran Polytechnic) Tehran, Iran.

3 Department of Biology and Anatomical Sciences, School of Medicine, ShahidBeheshtiUniversity of Medical Sciences, Tehran,Iran.

4 Department of Immunology, School of Medicine, Shahid BeheshtiUniversity of Medical Sciences, Tehran, Iran.

5 Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

6 Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran,Iran.

7 Department of Immunology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.


In this study, we evaluated the effects of nanofiber and film polymers with doxycycline for treating a wound in a diabetic rat model. 108 male rats were divided into six groups, the control group, the diabetic control, and the groups were diabetic rats receiving different wound dressing. At the 3rd, 7th, and 14th days, macroscopic/histologic imaging and tissue sampling were performed. Tissues were analyzed for IL-1β, TNF-α, IL-10, TIMP-1, and MMP-2 by using ELISA. Dressings of chitosan, polyvinyl alcohol and doxycycline increased the rate of wound closure, the volume of collagen, dermal, and epidermis; in addition, it increased the number of fibroblasts and basal cell epidermis cells, vascular length, and decreased the number of neutrophil cells. Inflammatory cytokines and MMP-2 were decreased, and anti-inflammatory IL-10 and TIMP-1 were increased. It was ultimately attained that the combination of chitosan/ polyvinyl alcohol /doxycycline could be a useful dressing for the healing of diabetic wounds.

Graphical Abstract

Chitosan/PVA/Doxycycline Film and Nanofber Accelerate Diabetic Wound Healing in a Rat Model


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