Microencapsulation of Omeprazole by Lactobacillus acidophilus ATCC 4356 Surface Layer Protein and Evaluation of its Stability in Acidic Condition

Document Type : Research article


1 Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.

2 Department of Pharmaceutics, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

3 Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.


The present study introduces a novel method for encapsulation of the acid-labile drug called
Omeprazole using
Lactobacillus acidophilus (L. acidophilus) ATCC 4356 S-layer protein. Before
preparing the Omeprazole suspension, a series of preliminary studies were performed on the
Omeprazole powder. For this purpose, some parameters such as melting point, IR spectrum,
UV spectrum, and the particle size of Omeprazole powder were investigated. The size reduction
process was done in order to achieve an ideal formulation. Ultimately, the resulting powder had
an average particle size of 35.516 μm and it was almost uniform. After calculating the amount
of S-layer protein required for complete covering of drug particles, the effect of different factors
on the drug coating process was investigated with one factor at a time method. Then stability of
coated Omeprazole was evaluated in acetate buffer (pH 5). Finally, the maximum coat of drug
particles was determined using S- layer protein of
Lactobacillus acidophilus ATCC 4356 at 25 °C
for 2 h, shaking rate of 100 rpm and ratio of 2:1 for S-layer protein amount/Omeprazole Surface
in Tris hydrochloride buffer medium (50 mM, pH 8). The coating of Omeprazole by the S-layer
protein decreased the drug decomposition rate up to 2.223

Graphical Abstract

Microencapsulation of Omeprazole by Lactobacillus acidophilus ATCC 4356 Surface Layer Protein and Evaluation of its Stability in Acidic Condition


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