Antioxidant Activity and Hemocompatibility Study of Quercetin Loaded Plga Nanoparticles

Document Type: Research article

Authors

1 Bioengineering Department, Chemical and Metallurgical Faculty, Yıldız Technical University, Istanbul,Turkey.

2 Bioengineering Department, Chemical and Metallurgical Engineering Faculty, Yildiz Technical University, Istanbul, Turkey.

3 Bioengineering Department , Faculty of Chemical and Metallurgy, Yildiz Technical University, Istanbul,Turkey.

4 Molecular Biology and Genetics Department, Science and Letters Faculty, Yıldız Technical University, Istanbul, Turkey.

5 Medical Biology Department, Medicine Faculty, Altinbas University, Bakirkoy, Istanbul, Turkey.

6 Bioengineering Department, Engineering and Architecture Faculty, Konya Food and Agriculture University, Konya, Turkey.

Abstract

Quercetin (QU) is an important flavonoid compound presenting lots of biological activities, but its application has been limited due to its low aqueous solubility and instability. In this study, in which conducted to improve these properties of the quercetin, quercetin-encapsulated PLGA nanoparticles were prepared, characterized and evaluated for antioxidant and hemolytic activity. Nanoparticles were produced by single emulsion solvent evaporation method. Four different process parameters initial QU amount, PVA concentration, PVA volume, and initial PLGA amount, were investigated to obtain the nanoparticles which have minimum particle size and maximum entrapment efficiency. Synthesized nanoparticles were evaluated for particle size, entrapment efficiency, and reaction yield. Additionally, antioxidant properties and in vitro hemolytic activity of quercetin loaded nanoparticles with different particle size were also evaluated for the first time in the literature. The antioxidant activity results showed that, nanoparticles have different antioxidant activity, depending on the amount of quercetin release from nanoparticles at different particle sizes. The hemolytic activity results show that all nanoparticles exhibited favorable compatibility to red blood cells and no significant hemolytic effect was observed.

Graphical Abstract

Antioxidant Activity and Hemocompatibility Study of Quercetin Loaded Plga Nanoparticles

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