Raise up of Scopolamine In Hairy Roots Via Agrobacterium rhizogenes ATC15834 as Compared With Untransformed Roots In Atropa komarovii

Banihashemi, Ofelia; Khavarinejad, Ramazan Ali; Yassa, Narguess; Najafi, Farzaneh

doi:10.22037/ijpr.2019.13550.11710

Raise up of Scopolamine In Hairy Roots Via Agrobacterium rhizogenes ATC15834 as Compared With Untransformed Roots In Atropa komarovii

Document Type: Research article

Authors

¹ Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

² Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medicinal Sciences, Tehran, Iran.

³ Department of Plant Science, Faculty of Biological Science, Kharazmi University Tehran, Iran.

10.22037/ijpr.2019.13550.11710

Abstract

Atropa komarovii generates tropane alkaloids and three other compounds such as hyoscyamine. Racemate atropine and scopolamine (hyoscine) are the main alkaloids that have anticholinergic, antispasmodic and sedative agents. A proficient convention has been reported for the formation of transgenic Atropa komarovii by the use of Agrobacterium rhizogenes. Root culture by utilizing leaves explants that were contaminated by Agrobacterium rhizogenes ATCC 15834, a strain with the paired vector. The hairy roots after contamination for three weeks shaped specifically from the cut edges of leaves. The PCR intensification demonstrated that rol B genes of Ri plasmid of Agrobacterium rhizogenes were coordinated and communicated into the genome of changed hairy roots. Examination of HPLC demonstrated that hairy roots can produce scopolamine and hyocyamine and it was appeared that scopolamine content essentially expanded in changed roots and hyoscyamine extremely expanded in non-transgenic roots. According to the results it was perceived that the scopolamine content in hairy roots was raised significantly since it was compared to control roots. it was evidenced that hairy roots gather a great number of metabolites that have a commercial significance. Thus later on we can enhance efficiency for example by building up the biosynthetic route overexpression of gene codifying enzymes in the metabolic route for expanding valuable secondary metabolites in plant cures.

Graphical Abstract