The Assessment of Toxicity Characteristics of Cellular Uptake of Paramagnetic Nanoparticles as a New Magnetic Resonance Imaging Contrast Agent

Document Type: Research article


1 Department of Pharmaceutics and Pharmaceutical Nanotechnology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

2 Departmentof Medical Imaging, School of ParaMedicine, Urmia University of Medical Sciences, Urmia, Iran.

3 Department of Medical Physics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

4 Department of Medical Physics, School of Medicine, Urmia University of Medical Science, Urmia, Iran.


Nanoparticles are unique that enable many promising medical and technological applications in
their physical, and chemical properties. It is widely accepted that nanoparticles should be
thoroughly tested for health nanotoxicity, but a moderate risk analysis is currently prevented by a
revealing absence of mechanistic knowledge of nanoparticle toxicity. The purpose of this study
was to assess in-vitro cytotoxicity of Gadolinium oxide with diethylene glycol polymer (Gd2O3-
DEG) and magneto liposome nanoparticles (MLNs) in Hepa 1-6 cell lines as models to assess
nanotoxicity in-vitro. The effects of magnetic nanoparticles on these cell lines were evaluated by
light microscopy and standard cytotoxicity assays. The underlying interactions of these
nanoparticles with physiological fluids are key characteristics of the perception of their biological
efficacy, and these interactions can perhaps be performed to relieve unpleasant toxic effects.Our
results demonstrated that the Gd2O3-DEG and MLNs had significantlydifferent non-cytotoxic
effects. Our results suggest that these cell lines provide valuable models to assess the cytotoxicity
of nanoparticles in-vitro. The results of the present study demonstrated that MLNs and Gd2O3-
DEG with lower longitudinal relaxation time (T1) than Gadolinium Pentetic acid (Gd-DTPA) in
Hepa 1-6 cell lines are sensitive positive Magnetic Resonance Imaging (MRI) contrast agents that
could be as attractive as candidates for cellular and molecular lipid content targets such as liver
diagnostic applications. These data reveal that MLNs is a useful positive contrast agent for
targeting and cell tracking. This will help to image of cells and special organs like liver that uptakes
liposomal formulation very well.


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