Hydro-Ethanolic Extract of Portulaca Oleracea Ameliorates Total and Differential WBC, Lung Pathology and Oxidative Biomarkers in Asthmatic Rats

Document Type : Research article

Authors

1 Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

2 Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

3 Natural Products and Medicinal Plants Research Centre, North Khorasan University of Medical Sciences, Bojnurd, Iran.

4 Department of Pathology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

5 Clinical Research Unit, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

Abstract

Objective: The effects of Portulaca oleracea (P. oleracea; PO) on total and differential WBC count, and oxidant/antioxidant biomarkers in bronchoalveolar lavage fluid (BALF) as well as on lung pathology in asthmatic rats were examined.
Methods and Methods: Rats were randomly divided into; control group (C), asthma group, asthma groups treated with either P. oleracea (rats that received PO 1, 2 and 4 mg/ml) or dexamethasone 1.25 μg/ml (D), (n=8 in each group). Total and differential white blood cells (WBC) count, nitrite (NO2), nitrate (NO3), malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and thiol levels in rats BALF were evaluated and lung pathological features were studied.
Results: Total WBC count, eosinophil, neutrophil and monocyte percentages, levels of NO2, NO3, MDA in the BALF and most pathological scores in the lung were increased but lymphocyte percentage, SOD, CAT and thiol levels were decreased in the BALF of asthmatic animals (p Conclusion: Our results demonstrated the ameliorative effects of P. oleracea on total and differential WBC count and oxidant-antioxidant biomarkers levels in BALF as well as lung pathological features in asthmatic rats, which propose the usage of this extract as a preventive anti-inflammatory treatment against asthma.

Keywords


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