Abedi-Gaballu, F., Abbaspour-Ravasjani, S., Mansoori, B., Yekta, R., Hamishehkar, H., Mohammadi, A., Dehghan, G., Shokouhi, B., Ghahremani Dehbokri, S., Baradaran, B. (2019). Comparative of in vitro evaluation between erlotinib loaded Nanostructured lipid carriers and liposomes against A549 lung cancer cell line. Iranian Journal of Pharmaceutical Research, 18(3), 1168-1179. doi: 10.22037/ijpr.2019.1100775
Fereydoon Abedi-Gaballu; Soheil Abbaspour-Ravasjani; Behzad Mansoori; Reza Yekta; Hamed Hamishehkar; Ali Mohammadi; Gholamreza Dehghan; Behrooz Shokouhi; Shaho Ghahremani Dehbokri; Behzad Baradaran. "Comparative of in vitro evaluation between erlotinib loaded Nanostructured lipid carriers and liposomes against A549 lung cancer cell line". Iranian Journal of Pharmaceutical Research, 18, 3, 2019, 1168-1179. doi: 10.22037/ijpr.2019.1100775
Abedi-Gaballu, F., Abbaspour-Ravasjani, S., Mansoori, B., Yekta, R., Hamishehkar, H., Mohammadi, A., Dehghan, G., Shokouhi, B., Ghahremani Dehbokri, S., Baradaran, B. (2019). 'Comparative of in vitro evaluation between erlotinib loaded Nanostructured lipid carriers and liposomes against A549 lung cancer cell line', Iranian Journal of Pharmaceutical Research, 18(3), pp. 1168-1179. doi: 10.22037/ijpr.2019.1100775
Abedi-Gaballu, F., Abbaspour-Ravasjani, S., Mansoori, B., Yekta, R., Hamishehkar, H., Mohammadi, A., Dehghan, G., Shokouhi, B., Ghahremani Dehbokri, S., Baradaran, B. Comparative of in vitro evaluation between erlotinib loaded Nanostructured lipid carriers and liposomes against A549 lung cancer cell line. Iranian Journal of Pharmaceutical Research, 2019; 18(3): 1168-1179. doi: 10.22037/ijpr.2019.1100775
Comparative of in vitro evaluation between erlotinib loaded Nanostructured lipid carriers and liposomes against A549 lung cancer cell line
1Immounology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
2Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
3Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
4Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
5Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
6Department of Pathology, Tabriz University of Medical Sciences, tabriz, Iran.
7Immounology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
8Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Abstract
Erlotinib (ELT) as a small molecule with poor solubility, poor bioavailability, and instability in gastrointestinal environment, has been considered as a therapeutic agent for Non-Small-Cell Lung Cancer (NSCLC) therapy through oral administration. In the present study, ELT-liposome and ELT-NLCs were successfully prepared and characterized by assessment of the particle size, zeta potential (ZP), polydispersity index (PDI), encapsulation efficiency (EE) and drug loading (DL). DAPI staining and Flow cytometry techniques were employed to probe anticancer activities of the optimal formulations. The obtained results indicated that the average size of optimized ELT-NLCs was 109±2 nm, while the optimal formulation of ELT-liposome was 130±4 nm. In addition, the values of EE, DL and cellular uptake were higher in ELT-NLCs than ELT-liposome. Moreover, the stability of ELT-NLCs and ELT-liposome were not significantly changed (P > 0.05) within storage time. The results of anti-cancer assessment showed that ELT-NLCs caused more cell viability reduction than ELT-liposome and free ELT. According to the Flow cytometry and DAPI staining results, the exposed A549 cells with ELT-NLCs had more rate of apoptosis than ELT-liposome. The obtained data from this study clearly showed that ELT-NLCs had better anti-cancer activity than ELT-liposome, which may be is related to the effective nano particle size, PDI, EE, and DL of ELT-NLCs.
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