Cardioprotective effect of ethanolic leaf extract of Melissa officinalis L against regional ischemia-induced arrhythmia and heart injury after five days of reperfusion in rats

Document Type: Research article


1 Razi Herbal Medicines Research Center Lorestan University of Medical Sciences Khorramabad, Iran. Cardiovascular Research Center, Shahid Rahimi Hospital, Lorestan University of Medical Sciences, Khoramabad, Iran.

2 Department of Physiology, School of Medicine, Tehran University of Medical Science, Tehran, Iran.

3 Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Sciences, Shahrekord, Iran.

4 Razi Herbal Medicines Research Center and Department of Physiology, Lorestan University of Medical Science, Khorramabad, Iran.

5 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad


Melissa officinalis has antioxidant and anti-inflammatory activities and is used in various diseases.
Aim of the study: We investigated the role of M. officinalis extract (MOE) against ischemia-induced arrhythmias and heart injury after five days of reperfusion in an in-vivo rat model of regional heart ischemia.
The leaf extract of M. officinalis was standardized through HPLC analysis. Adult male rats were subjected to 30 min of ischemia by occlusion of the left anterior descending coronary artery followed by 5 days of reperfusion. The rats were randomized to receive vehicle or M. officinalis as follows: group I served as saline control with ischemia, groups II, III and IV received different doses of MOE- (intragastric, 25, 50 or 100 mg/kg respectively), by oral gavage daily for14 days prior to ischemia
Administration of M. officinalis significantly improved ischemia/reperfusion (I/R)-induced myocardial dysfunction by reduction of infarct size, episodes of ventricular tachycardia(VT) and ventricular ectopic beat(VEB and duration of VT as compared to control group, stabilized ST segment changes, QTc shortening, increased the R and T wave amplitudes and increased the heart rate during ischemia, increased the serum superoxide dismutase (SOD) activity and decreased serum lactate dehydrogenase (LDH), Troponin I (CTnI) and malondialdehyde (MDA) levels, 5 days after reperfusion. MOE-100mg/kg was the effective dose. Cinamic acid (21.81±1.26 mg/gr) was the main phenolic compound of plant sample.
The ethanol extract of M. officinalis was observed to exhibit cardioprotective effects against I/R injury, probably due to antioxidant properties.


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