Purification of Sesquiterpenes from Saussurea LappaRoots by High Speed Counter Current Chromatography

Document Type : Research article


1 Pharmaceutical and Biological Engineering College, Chongqing University of Technology, Chongqing 400054, China.

2 College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400030, P.R. China.

3 Defense Key Disciplines Lab of Novel Micro-nano Devices and System Techonlogy, Chongqing, 400030, P.R. China.

4 International Research and Development Center of Micro-nano Systems and New Materials Technology, 400030, P.R. China.


Sesquiterpenes lactones including costunolide and dehydrocostus lactone, were isolated from Saussurea lappa roots, which had exhibited a wide range of biological activities such as anti-cancer, anti-inflammatory, antiulcer, and immunomodulatory activities. High-speed counter-current chromatography (HSCCC) was applied for the rapid preparative isolation of sesquiterpenes lactones from Saussurea lappa roots. A solvent optimization method for HSCCC was presented, i.e. the separation factors of compounds after the partition coefficient (K) of solvent system should be investigated. Using this method, 150 mg of costunolide, 140 mg of dehydrocostus and 15 mg of 10-methoxy-artemisinic acid with purities of 95%, 98%, and 98% were obtained within 150 min. The structures of three compounds were identified by mass spectrum (MS) and nuclear magnetic resonance (NMR) spectroscopy. These results offered an efficient strategy for separation of potentially health-relevant phytochemicals from Saussurea lappa roots.


Main Subjects

Chinese Pharmacopoeia Commission. Pharmacopoeia
of the People′s Republic of China, Volume I. People′s
Medical Publishing House, Beijing (2010) 312.
Cho JY, Baik KU, Jung JH and Park MH. In-vitro antiinflammatory effects of cynaropicrin, a sesquiterpene
lactone, from Saussurea lappa. Eur. J. Pharmacol.
(2000) 398: 399-407.
Peng Z, Wang Y and Fan J. Costunolide and
dehydrocostuslactone combination treatment inhibit
breast cancer by inducing cell cycle arrest and apoptosis
through c-Myc/p53 and AKT/14-3-3 pathway. Sci.
Rep. (2017) 7: 41254.
Li AF, Sun AL and Liu RM. Preparative isolation and
purification of costunolide and dehydrocostuslactone
from Aucklandia lappa Decne by high-speed countercurrent chromatography. J. Chromatogr. A (2005)
1076: 193-7.
Li GX, Wang YL, Li XM, Chen GG and Wei P.
A preparation method of high purity dehydrocostus
lactone and costunolide by HSCCC. West China J.
Pharm. Sci. (2009) 24: 526.
Sun YS, Lin HM, Liu ZB, Yang SF and Wang JH.
Preparative separation of minor bioactive compounds
from flower of P. cuspidatum by high-speed countercurrent chromatography and comparison of their
antioxidant activity. Sep. Purif. Technol. (2014) 134:
Chen L, Xin XL, Lan R, Yuan QP, Wang XJ and
Li Y. Isolation of cyanidin 3-glucoside from blue
honeysuckle fruits by high-speed counter-current
chromatography. Food Chem. (2014) 152: 386–90.
Wang DJ, Fang L, Wang X, Qiu JY and Huang
LQ. Preparative Separation and purification of
sesquiterpenoids from Tussilago farfara L. by highspeed counter-current chromatography. Quim. Nova
(2011) 34: 804-7.
Liu Y L, Wang P, Chen T, Jia J and Sun J. One-step
isolation and purification of four xanthone glycosides
from tibetan medicinal plant Halenia elliptica by
high-speed counter-current chromatography. Sep. Sci.
Technol. (2014) 49: 1119–24.
Cao K, Xu Y, Zhao TM and Zhang Q. Preparation of
sesquiterpenoids from Tussilago farfara L. by highspeed counter-current chromatography. Pharmacogn.
Mag. (2016) 12: 282-7.
Cao XL, Yang CL, Pei HR, Li XH, Xu XB and Ito Y.
HSCCC and its applications in medical research. J.
Sep. Sci. (2007) 9: 54-7.
Yoichiro Ito. Golden rules and pitfalls in selecting
Sesquiterpenes from S. lappa Roots by HSCCC
optimum conditions for high-speed counter-current
chromatography. J. Chromatogr. A (2005) 1065: 145-
Li J, Gao R, Zhao D, Huang X and Chen Y. Separation
and preparation of xanthochymol and guttiferone e
by high performance liquid chromatography and high
speed counter-current chromatography combined with
silver nitrate coordination reaction. J. Chromatogr. A
(2017) 1511: 143-8.
Zhang S, Li L and Cui Y. Preparative high-speed
counter-current chromatography separation of grape
seed proanthocyanidins according to degree of
polymerization. Food Chem. (2017) 219: 399-407.
Aneta SK, Joanna J and Sławomir W. Separation
of betacyanins from purple flowers of Gomphrena
globosa L. by ion-pair high-speed counter-current
chromatography. J. Chromatogr. A (2017) 1489: 51-7.
Cao K, Qian W, Xu Y, Zhou Z, Zhang Q and Zhang
XF. A new sesquiterpenoid from Saussurea lappa
Roots. Nat. Prod. Res. (2016) 30: 2160-3.
Dhilio RS, Kalsi PS and Singh WP. Guaianolide from
Saussurea lappa. Phytochemistry (1987) 26: 1209-10