Jahani, R., Mojab, F., Mahboubi, A., Nasiri, A., Tahamtani, A., Faizi, M. (2019). An In-Vivo Study on Anticonvulsant, Anxiolytic, and Sedative-Hypnotic Effects of the Polyphenol-Rich Thymus Kotschyanus Extract; Evidence for the Involvement of GABA-A Receptors. Iranian Journal of Pharmaceutical Research, 18(3), 1456-1465. doi: 10.22037/ijpr.2019.15579.13194
Reza Jahani; Faraz Mojab; Arash Mahboubi; Azadeh Nasiri; Armin Tahamtani; Mehrdad Faizi. "An In-Vivo Study on Anticonvulsant, Anxiolytic, and Sedative-Hypnotic Effects of the Polyphenol-Rich Thymus Kotschyanus Extract; Evidence for the Involvement of GABA-A Receptors". Iranian Journal of Pharmaceutical Research, 18, 3, 2019, 1456-1465. doi: 10.22037/ijpr.2019.15579.13194
Jahani, R., Mojab, F., Mahboubi, A., Nasiri, A., Tahamtani, A., Faizi, M. (2019). 'An In-Vivo Study on Anticonvulsant, Anxiolytic, and Sedative-Hypnotic Effects of the Polyphenol-Rich Thymus Kotschyanus Extract; Evidence for the Involvement of GABA-A Receptors', Iranian Journal of Pharmaceutical Research, 18(3), pp. 1456-1465. doi: 10.22037/ijpr.2019.15579.13194
Jahani, R., Mojab, F., Mahboubi, A., Nasiri, A., Tahamtani, A., Faizi, M. An In-Vivo Study on Anticonvulsant, Anxiolytic, and Sedative-Hypnotic Effects of the Polyphenol-Rich Thymus Kotschyanus Extract; Evidence for the Involvement of GABA-A Receptors. Iranian Journal of Pharmaceutical Research, 2019; 18(3): 1456-1465. doi: 10.22037/ijpr.2019.15579.13194
An In-Vivo Study on Anticonvulsant, Anxiolytic, and Sedative-Hypnotic Effects of the Polyphenol-Rich Thymus Kotschyanus Extract; Evidence for the Involvement of GABA-A Receptors
1Student Research Committee, Department of Pharmacology and Toxicology, School of Pharmacy, Shahid ‎Beheshti University of Medical Sciences, Tehran, Iran
2Department of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran,Iran.
3Food Safty Research Center, Department of Pharmaceutics, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
4Department of Pharmacology and Toxicology, School of Pharmacy, Shahid Beheshti University of Medical ‎Sciences, Tehran, Iran
5Department of Pharmacology and Toxicology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
6Pharmaceutical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Abstract
Antidepressant-like activity of T. kotschyanus has been recently reported by scientists but insufficient attention has so far been devoted to T. kotschyanus, and there is a lack of information on the other neurobehavioral effects and side effects of this species. In the current study, the anticonvulsant, anxiolytic and sedative-hypnotic, effects of Thymus kotschyanus extract on male NMRI mice were evaluated using pentylenetetrazole, maximal electroshock, elevated plus maze, and pentobarbital-induced sleeping tests. Since phenolic compounds and flavonoids have main roles in pharmacological effects of most plant extracts, the phenolic and flavonoid contents of the extract were measured with Folin-Ciocalteu and AlCl3 reagents. Acute toxicity, passive avoidance, and open field tests were carried out to assess the toxicity of the extract. To find out the possible mechanism of action, flumazenil as the specific GABAA receptor antagonist was used. Anticonvulsant and hypnotic effects of the extract were observed at 400 and 600 mg/kg. The extract at the dose of 200 mg/kg revealed significant anxiolytic effects, but it did not show any adverse effects on learning and memory at all the tested doses. Results of this study indicate that Thymus kotschyanus extract has anticonvulsant, anxiolytic and hypnotic effects, which are likely related to the ability of some phenolic compounds to activate α1-containing GABAA receptors but more experiments still need to be carried out in order to find the exact mechanism, active component, and the toxicity of the Thymus kotschyanus extract.
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