The Relaxant Effect of Crocin on Rat Tracheal Smooth Muscle and Its Possible Mechanisms

Document Type: Research article


1 Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

2 Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

3 Ardabil Imam Khomeini Educational and Clinical Hospital, Ardabil University of Medical Sciences, Ardabil, Iran.


Crocin, a component of saffron, showed hypotensive which is perhaps due to vascular smooth muscle relaxant effect. The relaxant effects of saffron on tracheal smooth muscle also could be due to its constituent, crocin. In the present study, the relaxant effects of crocin and its possible mechanisms on rat tracheal smooth muscle were investigated. The relaxant effects of three cumulative concentrations of crocin (30, 60, and 120 μM) or theophylline (0.2, 0.4, 0.6 mM) as positive control was examined on pre-contracted tracheal smooth muscle by methacholine or KCl in non-incubated or incubated conditions with different agents including atropine, chlorpheniramine, indomethacin, diltiazem, glibenclamide and propranolol. In non-incubated tracheal smooth muscle, crocin showed significant relaxant effects on KCl induced muscle contraction (p