Shi, Q., Wang, X., Liu, J., Xiang, X., Su, M., Huang, R., Song, C. (2019). Anti-Asthma effect of an active components group from decoction of Pheretima Aspergillum and its chemical composition characterized by Liquid chromatography-quadrupole time of flight mass spectrometry. Iranian Journal of Pharmaceutical Research, 18(2), 867-876. doi: 10.22037/ijpr.2019.1100666
Qingxin Shi; Xuanyuan Wang; Junyi Liu; Xingliang Xiang; Mingwu Su; Rongzeng Huang; Chengwu Song. "Anti-Asthma effect of an active components group from decoction of Pheretima Aspergillum and its chemical composition characterized by Liquid chromatography-quadrupole time of flight mass spectrometry". Iranian Journal of Pharmaceutical Research, 18, 2, 2019, 867-876. doi: 10.22037/ijpr.2019.1100666
Shi, Q., Wang, X., Liu, J., Xiang, X., Su, M., Huang, R., Song, C. (2019). 'Anti-Asthma effect of an active components group from decoction of Pheretima Aspergillum and its chemical composition characterized by Liquid chromatography-quadrupole time of flight mass spectrometry', Iranian Journal of Pharmaceutical Research, 18(2), pp. 867-876. doi: 10.22037/ijpr.2019.1100666
Shi, Q., Wang, X., Liu, J., Xiang, X., Su, M., Huang, R., Song, C. Anti-Asthma effect of an active components group from decoction of Pheretima Aspergillum and its chemical composition characterized by Liquid chromatography-quadrupole time of flight mass spectrometry. Iranian Journal of Pharmaceutical Research, 2019; 18(2): 867-876. doi: 10.22037/ijpr.2019.1100666
Anti-Asthma effect of an active components group from decoction of Pheretima Aspergillum and its chemical composition characterized by Liquid chromatography-quadrupole time of flight mass spectrometry
College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, Hubei, China.
Abstract
The decoction of Pheretima aspergillum is used as a traditional medicine for treatment of asthma in China with a long clinical history. The aim of the study was to investigate the anti-asthma activities of an active components group obtained from the decoction of Pheretima aspergillum in histamine and ovalubumin induced asthma guinea pigs. The incubation period of asthma, serum IFN-γ, IL-4 and LTB4 levels were tested and determined. As a result, the significant increase of asthma incubation period, serum IFN-γ level were observed in the asthma guinea pigs treated with the active components group. In addition, the serum IL-4 and LTB4 levels were obviously decreased compared to that of model controls. The chemical constituents of the active components group were identified or characterized using liquid chromatography-quadrupole time of flight mass spectrometry (LC-QTOF-MS/MS). Twenty oligopeptides were first identified or characterized in the active components group from the decoction of Pheretima aspergillum. It is reasonable to assume that the anti-asthma activities of Pheretima aspergillum mainly resulted from these oligopeptides. Also, synergistic effects among their compounds may exist in the anti-asthma activity of Pheretima aspergillum.
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